多发性硬化（MS）是中枢神经系统常见的脱髓鞘疾病，是青壮年致残的主要原因之一，目前尚缺乏有效的特异性免疫治疗手段。实验性自身免疫性脑脊髓炎（EAE）是MS的经典动物模型，该研究拟采用髓鞘少突胶质细胞糖蛋白（MOG）及完全弗氏佐剂建立EAE模型，分离发病期EAE小鼠脾脏树突状细胞（DC），在体外应用1,25-二羟维生素D3诱导这些DC成为耐受性DC，研究其细胞因子的分泌和细胞表面分子表型，并将这些耐受性DC回输给已经发病的EAE小鼠，观察1,25-二羟维生素D3处理的耐受性DC对EAE的治疗作用。并进一步研究其对CD4+CD25+调节性T细胞、CD8+CD28-抑制性T细胞、T细胞增生和凋亡、Th1/Th2细胞因子分泌的影响，从而阐明1,25-二羟维生素D3处理的DC对EAE治疗作用的机理，为耐受性DC特异性治疗MS提供新的方法和理论依据。

Multiple sclerosis is a common central nervos system(CNS) demyelinating disease,which is one of the marjor disease causing young disability and lack of efficient method of specific immunetherapy ，our study aim to explore specific immunotherapy of multiple sclerosis. In this study, we establish mice experimental autoimmune encephalomyelitis (EAE) animal model,then splenic dendritic cells are seperated from EAE mice.In vitro,dedritic cells are culutured with 1,25-dihydroxyvitamin D3 to induce tolerogenic dendritic cells. The phenotype and cytokines of tolerogenic DCs are well studied. To investigate whether 1,25-dihydroxyvitamin D3 induced tolerogenic DCs have the ablity to ameliorate EAE , the tolerogenic DCs are intravenous injected into EAE mice.Further study on the effect of tolerogenic DCs on the CD4+ CD25+ regulatory T cells, CD8+ CD28- inhibitory T cells, T cell proliferation and apoptosis and Th1/Th2 cytokines secretion are well conducted, so as to clarify the therapy mechanism of the tolerogenic DCs. This study is supposed to provide new method and the theoretical basis for the treatment of multiple sclerosis.