褪黑激素介导单色光影响鸡下丘脑能量平衡相关基因表达的作用机制

本课题组前期研究发现，与其他光组相比，单色绿光显著促进肉鸡骨骼肌生长和增重，提高血浆褪黑激素水平和饲料利用率。结果提示，单色光可能通过能量利用进而影响机体的生长发育，但作用机制不详。研究显示，下丘脑促食欲肽（NPY/AgRP）和抑食欲肽（POMC/CART）的相对平衡决定了机体的能量稳态。本项目拟通过松果体摘除阻断褪黑激素分泌，脑室内注射褪黑激素受体阻断剂、激动剂和TSHβ阻断剂，以及体外培养下丘脑神经细胞等方法，研究单色光对鸡下丘脑能量平衡相关基因表达的影响，确定褪黑激素在单色光影响下丘脑能量平衡相关基因表达中的作用，探讨褪黑激素介导单色光影响下丘脑能量平衡相关基因表达的受体途径及AMPK-mTOR胞内信号转导机制，以阐明褪黑激素介导单色光影响鸡下丘脑能量平衡相关基因表达的作用途径及分子机制。该结果将有助于合理利用光色信息提高家禽生产性能，为家禽饲养管理提供新的理论指导。

The previous study found that monochromatic green light promoted the growth of skeletal muscle and body weight gain, elevated the plasma melatonin levels and feed efficiency of broilers. The results showed that monochromatic light might affect the growth and development through energy utilization. However, the mechanism is not fully understood. Studies have shown that the relative balance of the orexigenic peptides (NPY/AgRP) and anorexigenic peptides (POMC/CART) in hypothalamus determine the energy homeostasis of the body. Using the methods of pinealectomy blocked the secretion of melatonin, intracerebroventricular injection of melatonin receptor blockers, agonists and TSHβ blocker, and cultivation of hypothalamic nerve cells in vitro, the effect of monochromatic light on the expression of energy balance related genes in hypothalamus will be studied, the role of melatonin in effect of monochromatic light on the expression of energy balance related genes will be determined, the melatonin receptor pathway and AMPK-mTOR signal transduction mechanism of melatonin mediated monochromatic light affected the expression of energy balance related genes will be discussed. Therefore, the pathway and molecular mechanism of melatonin mediated monochromatic light affected the expression of energy balance related genes will be elucidated. The results will be helpful to effective use of information of color of light to provide a new theoretical guidance in improving the productive performance in poultry.