花色苷的跨膜转运机制是个重要科学问题，目前有关果实花色苷合成调控机制的研究取得了重要进展，但花色苷进入液泡的跨膜转运机制却尚不明确。本项目在前期FaMATE8相关研究成果基础，以八倍体草莓作为研究对象，围绕FaMATE8介导的花色苷转运机制这一核心问题，通过等温滴定量热分析、构建酵母囊泡花色苷转运模型和基因瞬时过表达/RNAi沉默技术，从蛋白生物学特性、功能验证和转运分子机制三个层次来揭示FaMATE8介导的草莓花色苷转运机制及其生物学功能，并通过构建瞬时表达FaMATE8与RNAi沉默FaMATE8果实，揭示FaMATE8对采后草莓高二氧化碳贮藏响应机制及其对花色苷转运调控机制。研究结果不仅可丰富果实花色苷跨膜转运机制相关理论，也可为果实采后高二氧化碳保鲜技术的开发提供重要理论依据与技术支撑。

The transmembrane transport mechanism of anthocyanins is a vital scientific issue. In recent years, the regulatory mechanism of anthocyanin biosynthesis has been widely researched. However, the transmembrane transport mechanism of anthocyanins still reminded unclear. On the basis of our achievements about FaMATE8, we choose octaploid strawberries as the subject of this research. To understand the mechanism of anthocyanin transport mediated by FaMTAE8, isothermal titration calorimetry (ITC), vitro transport studies, transient overexpression/RNAi are applied in this research to clarify the biological characteristics, transport function and transport molecular mechanism of FaMATE8. Meanwhile, transient expression/RNAi fruit are used to reveal the response of FaMATE8 to elevated carbon dioxide during postharvest storage, and the regulatory mechanism of anthocyanin transport mediated by FaMTAE8 in postharvest as well. This research not only provides a theoretical basis for revealing transmembrane transport mechanism of anthocyanins, but also offers technical support for the application of elevated carbon dioxide in postharvest stoage.