ABA及其信号传导调控非跃变型果实成熟是一个重要科学问题，近年来发现miRNA通过与ABA信号通路上元件互补调控参与果实成熟，ABA信号下游转录因子及其反馈调控模式的研究未见报道。本项目在前期新发现Fan-miR73及靶向作用ABI5转录因子的工作基础上，以二倍体草莓为研究对象，探究Fan-miR73和ABI5在草莓果实成熟期间的表达模式，明确Fan-miR73和ABI5过表达或敲除对果实成熟的影响。采用EMSA、酵母单杂和ChIP-PCR等分子生物学手段，解析ABI5转录因子对Fan-miR73的反馈调控机制。利用ChIP-Seq和转录组学技术，挖掘ABI5转录因子下游调控基因。从而揭示Fan-miR73靶向作用ABI5转录因子调控草莓果实成熟中的分子调控机制。研究结果不仅为开发草莓等非跃变型果实采后保鲜方法提供技术支撑，也为揭示ABA信号传导调控非跃变型果实成熟机制提供理论依据。

ABA and its signal transduction are very important in regulating non-climacteric fruit ripening. MiRNAs were recently found to regulate fruit ripening by targeting components in ABA signaling pathway, however, transcription factors involved in the downstream of ABA signaling and feed-back regulatory mechanism have not been reported. This project which is based on the previous newly identified Fan-miR73 and its degradation effect on ABI5 mRNA will investigate the transcriptional levels of Fan-miR73 and ABI5 during wild strawberry (Fragaria vesca) fruit ripening, and it will also study the influences of over-expression or knock-out of Fan-miR73 or ABI5 on fruit ripening. We will explore the feed-back effect of ABI5 on Fan-miR73 by using methods of EMSA, yeast one-hybrid and ChIP-PCR. By taking advantages of ChIP-Seq and transcriptome technologies, we will further seek the downstream regulating genes of ABI5 transcription factor. The result may not only provide technical support for developing efficient preservation approaches for postharvest non-climacteric fruits, it will also illustrate the mechanism that how ABA signaling transduction regulates non-climacteric fruit ripening.