昆虫共生菌是害虫生物防治中一类重要微生物资源，明确昆虫与共生菌之间的互作机制、挖掘其中的关键基因资源以期利用是开发基于共生菌的害虫防控技术的重要基础和关键环节。本项目围绕“昆虫免疫耐受共生菌的分子机制”这一科学问题，以水稻重要害虫褐飞虱及其共生真菌为研究对象，以褐飞虱免疫调控因子Nlserpin1为研究切入点，综合运用生物化学、分子生物学以及功能基因组学等技术方法，解析Nlserpin1的生物学活性以及在褐飞虱与共生真菌免疫互作过程中的生物学功能，阐明其对褐飞虱免疫防御相关信号通路和防御相关基因的调控作用，结合互作蛋白的分离鉴定及互作关系剖析，构建Nlserpin1介导的免疫调控网络，从分子水平上揭示Nlserpin1调控褐飞虱免疫耐受共生真菌的作用机制。项目的实施和完成不仅为研究褐飞虱与共生菌互作机制奠定理论基础，也为开发基于共生关系调控的褐飞虱生物防治新技术提供科学依据和基因资源。

Insect endosymbiont is one of important microbial resources in the insect pest biocontrol. To clarify the interaction between insect and its endosymbionts and to excavate the valuable genes for utilizing are important foundations and key links in the development of endosymbiont-based pest control techniques. Focusing on the main aspects to reveal the molecular mechanism underlying the insect endosymbiont immunotolerance, this research uses the most important rice pests, Nilaparvata lugens (Brown planthopper, BPH) and its symbiotic fungus (Pichia guilliermondii) as the objects, and takes an immune regulatory factor Nlserpin1 in BPH as the cut-in point. Comprehensive application of methods and techniques in the biochemistry, molecular biology and functional genomics, the biological activity and functions of Nlserpin1 in the immunological interactions between BPH and its symbiotic fungus are clasified, and then its regulatory effects on the immune defense-related signaling pathways and immune defense-related genes in BPH are elucidated. Together with the research results of the interacting proteins identification and analysis, the Nlserpin1-mediated immune regulatory network are constructed. Combination of above all outcomes, the mechanism of Nlserpin1 on regulating fungal symbiont immunotolerance in BPH is consequently summarized at the molecular level. Implementation and completion of this project will not only lay the theoretical basis for the study of the interaction mechanisms between BPH and its endosymbionts, but also provide scientific foundation and genetic resources for developing BPH biocontrol techniques based on the symbiotic relationship between the microbe and its host insect.