基于光学投影层析技术的斑马鱼血流功能成像研究

本项目以光学投影层析技术为基础发展一种新型无标记的三维血流功能成像方法，用以研究斑马鱼等小动物的血液微循环系统发育和相关病理机制。项目以申报人已经实验验证并获得国际同行认可的运动红细胞在低相干光照明的条件下会产生吸收调制效应为物理基础；采用螺旋扫描的方式实现对长条型的成熟斑马鱼全尺寸成像；采用主成分分析（PCA）方法快速提取每个像素上静态、动态光信号，获得每个投影角度的光学血管造影图像，再通过滤波反投影算法实现3维的血流层析成像；在每个投影方向的血流图像中，采用图像互相关方法计算血流绝对速度（速度大小和速度方向），再用矢量叠加的方法实现各个投影方向的速度合成，获得三维血流速度测量。项目拟发展的技术对斑马鱼等小动物的循环系统的发育机制和血液动力学的机理研究有重要价值，具有很好的应用前景。

This project aims at developing a novel three-dimensional functional imaging technique for the study of the blood microcirculation development and the pathogenesis of zebrafish. Our Based on the absorption modulation effect illuminated by low-coherence light, which has been verified by our experiment and accepted by international peer. The imaging system is for the long striped zebrafish with helical scan recording; The principal component analysis is used to extract dynamic optical signal from the static one. Optical angiography of each projection angle is reconstructed, and then realize the three-dimensional vascular network is obtained by the back-projection algorithm. On this basis, 3D flow velocity image, containing speed value and direction, is obtained by introducing cross-correlation algorithm. The technology of the project is of great value to the development mechanism of circulatory system and the mechanism of hemodynamics of zebrafish, and it has a good application prospect.