在病原菌收集、分离、培养、鉴定、基因组测序以及甘蔗抗病性评价等研究基础上，完善不同地区、不同季节、不同寄主品种的甘蔗梢腐病的病原收集、分子鉴定、营养互补检测、遗传多样性分析以及致病力生物测定，建立甘蔗梢腐病病原资源库；利用全基因组酶切图谱技术对所测序的基因组进行补充、完善和验证，并定位到特定染色体上；通过比较基因组学解析引起甘蔗梢腐病病原菌的基因组结构和基因组成，特别是种属特异的染色体区；利用病原菌菌侵染甘蔗前后的转录组学分析，结合系统生物学和定量PCR结果，注释、鉴定和验证病原菌特异区的相关基因及其表达，阐明影响我国甘蔗梢腐病的病原菌种类分化及其关键基因，为深入研究甘蔗梢腐病病原菌的致病机理及其与甘蔗的分子互作和基因应答，设计环保高效杀菌剂的靶标基因以及甘蔗抗性品种的多系布局等提供理论依据。发表论文3篇、获得专利1项、培养博士1名、硕士3名。拟申请经费55万元。

Sugarcane pokkah boeng disease is one of the major sugarcane diseases, which resulted in the great loses of sugarcane yield in China. For controlling this disease, it is important to determine the pathogen and identify the pathogen-related genes that cause pokkah boeng disease. However, to date, there are no more reports on these researches. Therefore, based on pervious studies, including pathogenic collection, in vitro pure culture, pathogenic identification and genomic analysis, as well as resistant evaluation of sugarcane, our project will be focused on: 1) Investigating the space-time distribution of the pathogenic variations of pokkah boeng pathogen races in response to sugarcane production regions, host cultivars, pathogen ecotypes at the different growth and development stage using VCG analysis and molecular marker identification, and final establishing the pathogen library of sugarcane pokkah boeng disease; 2) Complementing, optimizing and confirming the sequenced genome, and locating the scaffords in specific chromosome using Optical mapping technology; 3) Discover the gene family and evolution of Fusarium moniliforme and to annotate the genomic structure, gene composition and metabolic in pathogen, especially lineage specific genomic region by comparative genomics analysis; 4) Annotating and identifying the pathogen-related genes and their expression in the lineage specific region of the pathogen by using transcriptome analysis on post-infection and preceding infection of the pathogen to sugarcane, coupled with system biology and real-time PCR. The outcome of this project will be to determine the pathogenesis of sugarcane pokkah boeng and its molecular interaction to sugarcane, to design target genes for efficient fungicide, and to evaluate the resistance of sugarcane cultivars for multi-lines cultivar layout in response to dominant physiological races or specialization types. It will take 4 years to complete this project, the outcome of this project will publish 2 SCI papers, to train 1 postdoctoral research associate, 1 Ph.D and 3 master candidates, and to apply 1 patents. Total of 580,000 RMB will be required to fund from NSFC.