Artificial Inoculation Method of Pokkah Boeng Disease of Sugarcane and Screening of Resistant Germplasm Resources in Subtropical China

Artificial Inoculation Method of Pokkah Boeng Disease of Sugarcane and Screening of Resistant Germplasm Resources in Subtropical China
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中国亚热带地区甘蔗小白病人工接种方法及抗性种质资源筛选

DOI:
10.1007/s12355-016-0465-7
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发表时间:
2017-06
期刊:
影响因子:
1.9
通讯作者:
Zhang MQ
Zhang MQ
中科院分区:
农林科学4区
文献类型:
--
作者:
Wang Ze-Ping;Sun Hai-Jun;Guo Qiang;Xu Shi-Qiang;Wang Ji-Hua;Zhang Mu-Qing;Wang Ze-Ping;Lin Shan-Hai;Zhang Mu-Qing;Wang Ze-Ping;Lin Shan-Hai;Zhang Mu-Qing;Zhang MQ;Zhang MQ;Zhang MQ

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评价四种不同的人工接种方法,注射器接种法、纺锤体接种法、离体叶片测定法和settdip法表明,注射器接种法最适合于在控制条件下评价甘蔗品种对PokkahBoeng病的抗性,其次是纺锤体接种法。然而,88份甘蔗种质资源对两种最流行的与博卡勃病有关的真菌的初步抗性评估,F。轮枝孢CNO-1和F. proliferatumYN-41,纺锤体接种法表明CNO-1的毒力较强。此外,我们的研究鉴定了GT 11、GT 37、NCO 310、POJ 2878和F134为高度敏感的,而CP 84 -1198、GT 05 -3846、ROC 27和YT 94 -128为高度抗性的克隆。纺锤体接种法是甘蔗苗期种质资源抗博卡病鉴定的最可靠的室内接种方法。
Evaluation of four different methods of artificial inoculation viz., syringe and spindle inoculation, detached leaf assay and sett dip methods indicated that the syringe method was the most suitable followed by spindle inoculation for evaluation of sugarcane varieties against pokkah boeng disease under controlled conditions. However, the preliminary assessment of the resistance of 88 sugarcane germplasm lines against two most prevalent fungal species associated with pokkah boeng disease,F. verticillioidesCNO-1 andF. proliferatumYN-41, by spindle inoculation method indicated that the CNO-1 was more virulent. Furthermore, our study identified GT11, GT37, NCO310, POJ2878, and F134 as highly susceptible, whereas CP84-1198, GT05-3846, ROC27, and YT94-128 as highly resistant clones. Spindle inoculation method was found as the most reliable method of inoculation of young sugarcane germplasm under indoor condition for screening resistance against pokkah boeng disease.
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