大豆高蛋白新基因的鉴定和功能标记的开发

蛋白资源的短缺是全球性严重问题，大豆作为植物蛋白质的主要来源，通过对其突变的高蛋白特异材料的研究获得优异的调控大豆蛋白的基因，进而提高大豆蛋白含量是切实可行的。本项目前期通过NaN3-60Coγ诱变获得了一个大豆高蛋白子叶折叠突变体，并已对其进行了初步定位和转录组测序的分析，进一步将通过精细定位筛选到高蛋白子叶折叠突变体的调控基因，另一方面利用262份大豆材料组成的自然群体进行全基因组关联分析，通过这两方面的结果，结合大豆基因组信息和生物信息学的方法挖掘高蛋白新的候选基因。进而通过对蛋白相关基因的功能研究，探讨大豆蛋白合成的分子机理。本研究将发掘大豆种质资源中优异的高蛋白种质和基因资源，并开发新的功能标记，有望通过分子标记辅助选择技术进行大豆高蛋白的种质资源创新和遗传改良。

The shortage of protein resources is a serious global problem. Soybean (Glycine max (L.) Merrill) is one of the most important plant protein sources. The potent ways to improve soybean protein content is the genetic engineering of the regulating the protein synthesis excellent genes containing by the research of the mutated high protein specific material. The high protein content and curled-cotyledon mutant hpcc of soybean was derived treated by NaN3 and 60Coγ rays. The candidate genes related to soybean protein content will be find by the fine gene mapping. On the other hand, we will carry out genome-wide association analysis through using a natural soybean population with 262 varieties. Through these ways, the candidate genes related to soybean high protein content will be identified by combining the soybean genome resources and bioinformatics. Through analyzing the biological roles for the genes involved in high protein content in soybean, this study aims to explain the molecular mechanism for protein synthesis. This project will explore the excellent soybean germplasm with high protein content and identify and develop new genes and functional markers, to improve the protein content in soybean through marker assistant selection.