GmAGL1调控大豆裂荚的分子机理研究

大豆炸荚是影响大豆产量的一个重要因素。通过基因芯片技术，本实验室从大豆中克隆了1个与荚皮开裂有关的MADS-box转录因子基因GmAGL1。mRNA表达分析表明GmAGL1在大豆花、荚等生殖器官中表达较高，在荚皮和雌蕊中的表达量也较高。GmAGL1在拟南芥中的异位表达导致了果荚提前开裂和无花瓣等现象。为进一步阐明GmAGL1在大豆荚皮开裂以及生殖发育过程中的作用机理，本研究拟通过大豆转基因技术获得GmAGL1基因过量表达和RNA干涉敲除的大豆转基因植株，结合表型鉴定和细胞学分析研究GmAGL1的生物学功能；利用基因芯片和染色质免疫共沉淀测序(ChIP-Seq)技术，鉴定GmAGL1的下游靶基因及其表达调控通路；通过酵母双杂交技术鉴定GmAGL1互作蛋白，并研究GmAGL1与其他调控因子的互作在大豆荚皮开裂过程中的调控机制,为大豆高产分子育种提供理论依据和新的材料。

Pod dehenscence is an important factor causing yield loss during the havest of soybean. A MADS-box gene (GmAGL1) was cloned from soybean. Sequence alignment showed that GmAGL1 belonged to AG subfamily of C/D class in ABCDE model, suggesting the functions of GmAGL1 on stamen and carpel identities and regulation of fruit development. Gene expression analysis showed that GmAGL1 was highly expressed in reproductive organs of soybean, including flower, pod and seed. GmAGl1 was also highly expressed in pod wall. In mature flower organs, GmAGL is mainly expressed in pistils. Ectopic expression of GmAGL1 in Arabidopsis caused early pod dehiscence and the loss of petals. To clarify the regulatory mechanism of GmAGL1 in pod dehiscence in soybean, transgenic soybean plants over-expressing or RNAi knock down expressing GmAGL1 will be generated. The biological roles of GmAGL1 will be characterized by phenotypical and cytological analysis of reproductive development and pod dehiscence in transgenic plants. Microarray, ChIP-sequcencing and yeast two hybrid technologies will be employed to identify the GmAGL1 target genes and the signal network regulated by GmAGL1 in regulating pod dehiscence in soybean.