大豆抗裂荚新基因和功能标记的挖掘

大豆炸荚是影响大豆产量的一个重要因素。大豆种质资源中蕴含了丰富的抗裂荚基因。为了高效发掘大豆中抗裂荚的优异新基因，本项目一方面利用219份大豆材料组成的自然群体进行全基因组关联分析，另一方面利用两个重组自交家系群体，其中一个为栽培大豆和野生大豆杂交的重组自交家系，进行主效QTL的精细定位。通过这两个手段结合大豆基因组信息和生物信息学等方法挖掘和克隆抗裂荚新的候选基因。该研究通过对抗裂荚基因的功能研究，探讨大豆裂荚的分子机理；同时发掘大豆种质资源中优异的抗裂荚种质和基因资源，开发新的功能标记，有望通过分子标记辅助选择技术进行大豆抗裂荚的种质资源创新和遗传改良。

The pod dehiscence is one of the important factors affecting the yield of soybean. Allelic genes for pod dehiscence tolerance are abundant in soybean germplasm. In order to identify the new genes with tolerance to pod dehiscence in soybean, the research on the one hand will carry out genome-wide association analysis through using a natural soybean population with 219 varieties. On the other hand, we will identify the major QTL loci through linkage mapping using two recombinant inbred lines (RILs) populations. Through these ways, the candidate genes related to soybean pod dehiscence will be identified by combining the soybean genome resources and bioinformatics. Through analyzing the biological roles for the genes involved in pod dehiscence tolerance, this study aims to explain the molecular mechanism for pod dehiscence. This project will explore the excellent soybean germplasm with pod dehiscence tolerance and identify and develop new genes and functional markers, to improve the pod dehiscence tolerance in soybean through marker assistant selection.