变应性鼻炎（AR）发病机理不明确，难以确定有效的生物治疗靶点。本研究拟（1）建立AR小鼠模型，致敏阶段和激发阶段通过Bregs相关抗体进行剔除Bregs或者通过过继转移Bregs，研究Bregs对小鼠鼻粘膜中嗜酸性粒细胞炎症的影响及对ILC2调控的相关分子或者细胞机制。（2）体外建立Bregs与ILC2共培养体系，抗体中和或封闭Bregs作用的相关分子，探讨Bregs对ILC2作用及作用的关键分子。（3）建立AR小鼠模型，致敏阶段和激发阶段过继输入从关键分子敲除后的小鼠脾脏分离的Bregs，体内探讨Bregs对ILC2作用及作用的关键分子。本研究通过对AR中Bregs对ILC2细胞的调控进行研究，这将有利于我们加深对AR发病机理的认识，为AR的治疗提供新的生物靶点。

The pathogenesis of AR remains unclear, it is difficult to get effective treatments for the disease.For the study, we aim (1) To establish an AR model to study the effect of Bregs on ILC2 with the depletion of Bregs or adopitive transfer of Bregs at sensitization stage and challenge stage respectively in vivo.(2) To establish the coculture system of Bregs and ILC2 in vitro and neutralize or block the related molecules on Bregs to explore the critical molecules that Bregs have when they have any effect on ILC2.(3)To establish the AR modelto study the regulation of Bregs on ILC2 through adoptive transfer of Bregs with the critical molcules knocked out at sensitization stage and challenge stage in vivo.The study aims to explore the regulation of Bregs on ILC2,which is profitable for us to have more knowledge of AR and make new and effective treatments for the disease.