银屑病是一种伴随饮食和生活方式变化而日趋常见的皮肤病，目前其临床治疗与研究的难点在于对其发病机理缺乏整体认识和鉴定关键的治疗靶标。本项目拟采用代谢组学的方法，从代谢所引起的微环境改变这一全新视野入手，运用气相（液相）质谱寻找银屑病差异小分子，并通过分子生物学的方法研究差异小分子对于Th17/Treg的激活、分化、趋化和角质形成细胞分泌细胞因子等过程的调控作用。并以CD147分子作为一个关键节点，以CD147和几种主要代谢产物之间的相互调控为一个反馈环路，研究其对于下游信号通路和STAT3、MYC、FOXs等重要转录因子的调控作用，进而检测这些转录因子调节的T细胞激活、代谢相关的基因表达情况。再通过皮肤、T细胞3维共培养和CD147转基因和敲除小鼠模型进行验证。最后通过对临床病例样本的免疫组化（皮损）、ELISA（血样）等实验，阐明在银屑病进程中，代谢的改变和CD147在其中所扮的角色。

Psoriasis is more and more common disease accompany with changes in diet and lifestyle. The most difficult problems of psoriasis on research and therapy are unsatisfied knowledge and identified therapeutic targets. This study, based on metabolomics methods, to find the microenvironment difference cause by metabolism small molecular, investigate those molecular effect in the Th17/Treg activation, differentiation, Chemotaxis, as well as keratinocyte secretion by using gas (liquid) chromatographic mass spectrometry and cell, mouse and patients’ samples. By take CD147 as a key point in this network, we will investigate the feedback loop between CD147 and those small molecular, and investigate the downstream signal pathway as well as transcriptional factors (TFs). Moreover, genes regulated by those TFs would be tested and investigated. The skin/T cell co-culture model would be used to verify the results got from cellular experiments. Finally, by immunohistochemistry and ELISA methods, we will check the key results in clinical patients’ samples. The most interesting goal of this project is that reveal the role of metabolism change and CD147 function in psoriasis development.