我国鱼类养殖业每年因病害造成的经济损失巨大。应用疫苗进行免疫防控是控制鱼类疫病发生最有效的措施。灭活疫苗是渔用疫苗的主要形式，但传统灭活处理会改变细菌表面抗原结构，使其免疫原性降低。申请人在前期研究中发现，半滑舌鳎TFPI-2 (tissue factor pathway inhibitor 2, TFPI-2) 的C末端衍生肽TC38（含38个氨基酸）在杀伤创伤弧菌的过程中不会破坏细胞膜，保留了较为完整的细菌空壳，初步免疫实验证实该细菌空壳具有良好的免疫保护效果。基于此，本项目提出用TC38制备针对其靶细菌的新型灭活疫苗，建立TC38制备新型灭活疫苗的方法，检测其免疫效果，优化疫苗的导入方式和免疫策略，解析疫苗诱发的免疫机制，明确疫苗对鱼体生长、鱼体肠道菌群和水体生态环境的安全性，并探索疫苗在鱼类病害防控中的应用潜能。本项目的实施将为灭活疫苗的研制和鱼类病害防治提供新的思路和途径。

The economic loss caused by fish diseases in China is very huge. Immunization of fish with vaccine is an effective measure for the prevention and control of disease. Inactivated vaccine is the main form of fish vaccine, but the traditional inactivation treatment changed the structure of bacterial surface antigen and thus reduced its immunogenicity. We found in our previous research, that TC38 (with 38 amino acids), a C terminal peptide derived from tongue sole TFPI-2 (tissue factor pathway inhibitor 2, TFPI-2), killed Vibrio vulnificus cells without lysis of the cell membrane. Subsequent immunological experiments showed that the bacteria shell is an effective vaccine with a higher protective potential. Based on these findings, in this study, we will firstly prepare the novel inactivated vaccine with TC38, then, explore the immune effect and the best strategy of immunization of the vaccine; furthermore, investigate the immune response mechanism induced by the vaccine; clarify the safety of the vaccine toward fish, fish intestinal bacteria and water ecological environment; finally, study the application of the vaccine in fish disease prevention. The success of this study will provide new ideas for the development of inactivated vaccine and the prevention and control of fish diseases.