昆虫多肽因分子量小，毒副作用低，活性高，可以通过提高免疫功能抑制肿瘤的生长和转移，已成为肿瘤治疗研究的一个新的热点。该项目拟运用本实验室从美洲大蠊中分离的已知具有抗肿瘤活性的小分子肽/多肽单体A1125，体外分析小分子肽对人体免疫细胞的作用，了解美洲大蠊小分子肽/多肽单体A1125作为免疫刺激剂的可能性以及小分子肽通过刺激免疫细胞的抗肿瘤作用。将抗肿瘤小分子肽/多肽单体A1125运用于MGC-803及K562小鼠荷瘤模型，采用培养、流式细胞术、RT-PCR、ELISA等方法分析该小分子肽对小鼠肿瘤局部的免疫作用，以及对荷瘤小鼠全身性适应性免疫及固有免疫的作用，综合评估美洲大蠊小分子肽/多肽单体A1125的免疫调节在抗肿瘤中的地位和作用，为该药物的开发及临床应用提供基础。

The polypeptides from insect become a new study hotspot of antitumor drug because they are small, high active molecules, and they have less side effects. They can inhibit the growth and metastasis of tumor throug improving the functions of immune system. In this project, the antitumor small peptides/A1125 will be isolated from Periplaneta americana by our laboratory's methods. We will detect the effects on the human peripheral blood mononuclear cells (PBMC) of the peptides in vitro to understand if the peptides can be as immune stimulatants and carry out antitumor effects of stimulating immune cells. After MGC-803 and K562 tumor cells are inoculated into mice, the mice with tumores will be treated with the peptides. Then the local immunity, adaptive and innate immunity will be test in the mice by FACS, RT-PCR, ELISA, and so on. The immune regulation of the peptides will be evaluated in antitumor to provide a basis for the peptides applying clinical treatment and development.