黑肩绿盲蝽是稻飞虱的重要捕食性天敌。我们前期研究发现亚致死浓度三唑磷、溴氰菊酯、吡虫啉、阿维菌素等杀虫剂处理诱导黑肩绿盲蝽生殖，但相关的作用机制尚不清楚。初步研究结果表明胰岛素信号通路参与了杀虫剂诱导的黑肩绿盲蝽生殖调控。本项目以三唑磷、阿维菌素为供试药剂，以黑肩绿盲蝽为研究对象，首先研究亚致死浓度杀虫剂对黑肩绿盲蝽胰岛素信号通路关键基因（InR, PI3K, Akt）时空表达动态和磷酸化的影响；再采用分子生物学、昆虫行为学、免疫组织化学等方法，研究胰岛素信号通路关键基因对杀虫剂处理的黑肩绿盲蝽生殖力、种群增长、生殖行为、卵黄形成、卵子发生的调控作用，以揭示胰岛素信号通路对杀虫剂诱导黑肩绿盲蝽生殖的调控机制。本研究可为阐明农药对天敌昆虫生殖刺激的激素和分子调控机制提供理论依据；同时为选择合适的农药（有利于天敌生殖）与天敌协同防治害虫提供指导。

Cyrtorhinus lividipennis is a predominant predator of the rice planthoppers. Our previous study indicated that insecticides triazophos, deltamethrin, imidacloprid and avermectin could induced the reproduction of Cyrtorhinus lividipennis. Furthermore, we found insecticide treatment increased the relative expression level of genes in insulin signaling pathway and RNAi of the gene caused the reduction of fecundity of C. lividipennis, which indicated that insulin signaling pathway had function in the insecticide-induced reproduction of C. lividipennis. In this project, we would firstly investigate the effect of insecticides (triazophos and avermectin) treatment on the levels of expression and phosphorylation of the key genes (InR, PI3K, Akt) related to insulin signaling pathway in the predator. Subsequently, using molecular, behavioral, and immunohistochemical technologies, we would examine the regulatory function of key genes related to insulin signaling pathway on the reproduction capability, population growth, reproductive behavior, vitellogenesis and oogenesis in insecticide-treated C. lividipennis. Our results will provide valuable information for improving our understanding of the mechanisms of insecticide-induced reproduction regulated by hormonal signaling molecular in C. lividipennis. This study will also help us to select appropriate insecticides (e.g. by stimulation of reproduction of natural enemies) having high compatibility with C. lividipennis to control rice pest insects.