本研究在课题组完成绒山羊胚胎期毛囊发生发育不同阶段皮肤组织microRNA和mRNA高通量测序的基础上，对microRNA和mRNA数据进行联合信息分析，结合内蒙古绒山羊胚胎期毛囊形态发生发育变化规律的组织学研究结果，筛选与次级毛囊发生发育相关的microRNA及其作用的靶基因。通过实时定量PCR技术检测microRNA在内蒙古绒山羊胚胎期不同阶段的表达情况，确认与次级毛囊发生发育相关的microRNA。通过实时定量PCR技术和构建荧光素酶报告基因系统对重要microRNA的候选靶基因进行验证。合成重要microRNA的mimics和inhibitors，转染体外培养的内蒙古绒山羊真皮成纤维细胞、表皮细胞，检测细胞中靶基因及毛囊发生发育相关信号通路基因mRNA和蛋白质表达变化情况，揭示绒山羊胚胎期次级毛囊发生发育分子调控作用，为绒山羊绒毛生长分子调控机理奠定基础。

On the basis of microRNA and mRNA high-throughput sequencing at different embryo stages in Inner Mongolia cashmere goat, the integrative analysis of mRNA and microRNA will be performed in this study. Combined with histological research results of hair follicles morphogenesis and development at different embryo stages of Inner Mongolia cashmere goat, the related microRNAs and their target genes will be screened. In order to confirm microRNA associated with morphogenesis and development of secondary hair follicle, the expression of microRNA in embryo skin of different stages will be detected by real time quantitative PCR.The candidate target genes will be verified by real time quantitative PCR and the luciferase reporter gene system. Mimics and inhibitors of important microRNAs will be synthesized and transfected into cultured dermal fibroblasts and epithelial cells of Inner Mongolia cashmere goat in vitro. The changes of mRNA and protein expression of the target gene and the signal pathway gene of hair follicle morphogenesis and development were detected,which could reveal molecular regulation of secondary hair follicle morphogenesis and development at embryo stages in cashmere goat. All of these will lay foundation for molecular mechanism of cashmere goat hair growth.