基于课题组近年在内蒙古绒山羊胚胎期毛囊形态发生、发育规律研究方面取得的成果，结合绒山羊生产需求，以我国特有遗传资源内蒙古绒山羊为研究对象，开展胚胎期次级毛囊形态发生、生长发育相关基因分子的调控机理研究，为加快绒山羊遗传育种进展奠定理论基础。本研究在课题组完成绒山羊胚胎期45天、55天、65天皮肤毛囊转录组和蛋白质组测序的基础上，开展转录组数据和蛋白质组数据的联合信息分析，发掘出次级毛囊发生、发育重要的相关基因作为候选基因；运用实时定量PCR、原位杂交技术检测候选基因在次级毛囊不同发育时期的表达模式，进一步确认次级毛囊发生发育相关候选基因；通过Western Blot技术检测候选基因蛋白水平的表达模式；构建候选基因过达载体，转染体外培养的绒山羊真皮成纤维细胞、毛乳头细胞，探索候选基因在次级毛囊发生、发育相关信号通路基因间的作用关系。揭示候选基因在绒山羊次级毛囊形态发生、生长发育中的作用机制。

Based on the research achievements in recent years about morphogenesis and development of hair follicle in the Inner Mongolia Cashmere goat (Capra hircus) embryo stage, and with the goat production demand, we take Inner Mongolia Cashmere goat as the research object which is unique genetic resources of CHINA, launch the research about related gene’s molecular regulation mechanism of secondary hair follicle morphogenesis, growth and development in embryo stage, and lay a theoretical foundation for speeding up the progress of genetic breeding of cashmere goats. In this study, we continue the research on the basement of the finished achievement about skin hair follicle transcriptome and proteome sequencing of 45 days, 55 days and 65 days embryo, and work on the analysis of the transcriptome data associate with proteome data, from the data analyze we find the important genes which is related secondary follicle morphogenesis and development as candidate genes. Using the method of real-time PCR and in situ hybridization to detect the candidate genes expression patterns in different development period of the secondary hair follicle and further confirm the candidate genes which is truly related with development of secondary hair follicle . Using the method of western blot to detect the proteins expression level of the candidate gene. By construct the candidate genes overexpression vector, and transfect it into cultured cashmere goat dermal fibroblasts cell and dermal papilla cells in vitro, to explore the candidate gene work effection relationship in the signaling pathways of the occurrence and development of secondary hair follicle . In summary, this project will reveal the regulatory mechanism of the candidate genes in morphogenesis and development of secondary follicle in cashmere goat .