免疫吸附技术是目前实验室免疫筛查分析最常用的方法之一，在食品安全、临床诊断和环境监测等领域发挥着极其重要的作用。然而传统酶联免疫吸附法存在因比色信号强度弱导致的检测灵敏度偏低、以及因生物酶稳定性差导致的方法鲁棒性不足等问题。本研究拟基于巯基配体金纳米簇的聚集发光增强原理，结合无机催化剂介导的信号放大机制来构建高灵敏、高鲁棒性的无酶免疫检测方法，并实现食品中三种真菌毒素（黄曲霉毒素B1,赭曲霉毒素A和玉米赤霉烯酮）的高灵敏检测。首先，在巯基配体金纳米簇表面分别修饰叠氮基和端炔基两种点击化学基团；然后基于铜催化叠氮炔环加成反应介导金纳米簇聚集，构建基于无机催化剂介导聚集诱导发光原理的新型信号输出体系；最后在包覆支化聚乙烯亚胺的二氧化硅纳米颗粒表面原位还原Cu(II)，制备可介导金纳米簇聚集的无机非均相纳米催化剂，进一步以其为载体制备竞争抗原构建新型竞争免疫荧光分析技术。

Immunosorbent assay is one of the most popular immunological screening technique which has been widely used in food safety, clinical diagnosis and environment monitoring. While traditional enzyme labeled immunosorbent assay (ELISA) suffers from low detection sensitivity due to its low intensity of colorimetric signal and poor robustness arising from the poor stability of natural enzymes. Herein, an ultrasensitive and robust enzyme-free immunoassay is proposed for the detection of three mycotoxins in food (aflatoxin B1, ochratoxin A and zeaxanone), where aggregation induced enhanced emission of thiol ligands protected gold nanoclusters (AuNCs) is used as a signal output, with a signal amplification based on an inorganic catalyst involved reaction. Firstly, thiol ligands AuNCs are modified with azide groups and alkyne groups separately. And then, a novel signal transition mechanism based on aggregation induced emission amplified with inorganic catalyst is established, by using Copper-catalysed azide–alkyne cycloadditions to trigger the aggregation of AuNCs modified with clickable groups. Then a polyethyleneimine (PEI) mediated in-situ reduction of Cu(II) ion on the surface of silica nanoparticles would be conducted to prepare Silica@PEI@CuNPs nanoparticle, which can be used as a heterogeneous catalyst and a carrier of competitive antigens for the novel proposed fluorescence immunoassay.