体外模拟胶原矿化及仿生制备骨组织工程材料是生物材料领域的研究热点，矿化液中胶原的自组装是关键控制步骤。目前关于胶原、非胶原蛋白（NCPs）、多糖和羟基磷灰石（HAP）之间的相互作用尚不清楚且存在争议。本项目基于胶原在溶液中的聚集行为特点，将胶原自组装、矿化胶原纤维性质与矿化液中胶原的聚集态结构相关联，以此实现对胶原矿化过程以及胶原/HAP复合材料性能的调控。拟采用荧光、光散射等技术研究矿化液中胶原的聚集态结构；采用浊度法等方法研究胶原的自组装动力学；采用能谱、X射线衍射等手段研究HAP的晶体结构形态；采用电镜、热分析等技术研究矿化胶原纤维的结构与性能；阐明矿化液中胶原的聚集态结构、自组装以及矿化胶原纤维结构性能的关系，揭示NCPs分散剂、多糖对胶原纤维矿化的调控机制。本项目研究对于骨组织工程材料的仿生制备以及骨骼的再生与修复都具有重要的理论意义和应用价值，也为生物矿化理论提供新的实验依据。

In vitro modeling of collagen mineralization and the biomimetic preparation of bone tissue engineering materials is a hot topic in the field of biomaterials and the self-assembly of collagen in mineralized solutions is the key control procedure. The interactions among collagen, non-collagen proteins (NCPs), polysaccharides and hydroxyapatite (HAP) are both unclear and controversial. Based on the aggregation behavior of collagen in solution, the self-assembly of collagen and the properties of mineralized collagen fibers were correlated with the aggregation structure of collagen in solution, so as to realize the regulations of the collagen mineralization process and the performance of collagen/HAP composites. The project is to study the aggregation structure of collagen in mineralized solution by fluorescence and light scattering etc. Moreover, the self-assembly kinetics of collagen was studied by turbidity method etc. The structure and morphology of HAP were studied by means of energy spectrum and X-ray diffraction etc. The structure and properties of mineralized collagen fibers were studied by means of electron microscopy and thermal analysis etc. The relationship among the aggregation structure of collagen in mineralized solution, collagen self-assembly and the structure and properties of mineralized collagen fibers was elucidated. The regulation mechanism of the NCPs dispersants and polysaccharides on the mineralization of collagen fibers was disclosed. The project has important theoretical significance and application value for the biomimetic preparation of bone tissue engineering materials and the regeneration and repair of bone, and also provides a new experimental basis for biomineralization theory.