弯曲菌是一种重要的食源性致病菌，广泛定植于动物肠道中，可通过食物链传播给人引起一系列胃肠道疾病。大环内酯类药物是治疗弯曲菌感染的特效药物，而近年来我国动物源弯曲菌对大环内酯类药物的耐药率不断上升，给公共卫生安全带来巨大威胁。目前，携带核糖体甲基化酶基因erm(B)的多重耐药基因岛是弯曲菌中介导大环内酯类耐药性水平转移的主要机制。我国动物源erm(B)阳性弯曲菌的流行率已呈上升趋势，但导致其传播扩散的机制仍未彻底阐明。因此，本研究拟运用生物信息学和动物试验等方法，通过明确弯曲菌erm(B)耐药基因岛的分子传播机制、进化特征及水平转移能力，阐明erm(B)耐药基因岛的传播机制；通过探明erm(B)阳性弯曲菌优势克隆株的遗传进化规律与适应性能力，结合erm(B)耐药基因岛的传播机制全面揭示erm(B)阳性弯曲菌流行的规律。研究成果可为控制动物源耐药弯曲菌的传播和风险评估提供科学依据和理论指导。

Campylobacter spp., which are widely colonized in animal intestinal tract, are major foodborne pathogens and leading bacterial causes of gastroenteritis through food chain. Macrolides are considered as the special drug of choice for therapeutic purposes, while the macrolide resistance rate has increased continuously in recent years, which pose a great threat on public health security. Multidrug genomic island carried the ribosomal methylation encoded by erm(B) is leading horizontally transferrable resistance mechanisms in Campylobacter. The prevalence of erm(B)-positive Campylobacter from animal origin in China is increasing, while the reason is still unclear. Thus, based on bioinformatics technology and animal experiments, the aim of this study is to illuminate the transmission mechanism of erm(B)-carried resistant genomic island by means of molecular transmission mechanism, evolutionary characteristics and horizontal transfer capacity. Meanwhile, we also aim to clarify the epidemic pattern of erm(B)-positive Campylobacter through the direction like the evolution and fitness. Findings in this study will provide scientific basis for the risk assessment and resistance containment to control the dissemination of resistant Campylobacter from animal origin.