吗啡撤退诱发的负性情感是长期慢性使用吗啡个体复吸的主要动机，研究吗啡撤退负性情感的调控机制对于治疗戒毒后复吸显得尤为重要，目前知道杏仁核是调节焦虑、恐惧等负性情绪反应的主要脑区，推测CEA对吗啡撤退负性情感诱发的复吸起着重要的调控作用。本项目旨在利用不同亚型GABA神经元Cre 转基因小鼠和loxp-腺相关病毒，结合吗啡撤退诱发的小鼠条件性位置厌恶，高架十字迷宫和糖水主动摄取行为，对杏仁核不同类型抑制性神经元在吗啡撤退负性情感及其诱发的复吸中的作用进行研究，并利用光遗传学操控的电生理技术和免疫荧光探讨其发挥调控作用的神经环路和生物学机制。本研究将促进对药物戒断后复吸相关神经环路和生物学机制的了解，为药物戒断后复吸的防治提供理论依据和干预新思路。

The affective states of morphine withdrawl powerfully motivate drug-seeking behaviour and may trigger relapse to morphine, therefore exploring the regulation mechanism of morphine withdrawl induced affective states is very important to relapse. At present only known central of amygdala(CEA) is the main brain area of regulating fear and anxiety related affective states, thus speculate CEA may participate in regulating the relapse induced by the affective states of morphine withdrawl. Therefore this project aims to use different subtype GABAergic neuron-Cre mice and loxp-AAV system,combined with morphine withdrawl induced mice conditional place aversion behavior,the elevated pulse maze and sweet water self-administration to research the function of different subtype neurons of CEA in the affective states of morphine withdrawl and it-induced relapse.And use optogenetics,in vitro electrophysiology and immunology to explore its neural circuits and neurobiology mechanism.This research will accelerate the understanding of the neurocircuits and neurobiology mechanisms of relapse after morphine withdrawl, then provide the indications and theoretical basis for clinical interventions to drug relapse.