内生真菌在植物对抗环境压力和增加生物量等方面起着重要作用。陆生植物的内生真菌已有相关研究，但分布更为广泛的水生植物的内生真菌却没得到关注。少量的研究结果显示，相对于陆生植物，水生植物的内生真菌种类独特，物种丰富，在水生植物抵抗环境压力方面也发挥着重要作用。而且水生植物在检测地理格局和环境梯度对生物多样性的影响方面是一个好的案例，对水生植物内生真菌分布格局的研究也将丰富生物多样性研究的内涵。. 本项目旨在前期研究的基础上，选择中国西南地区水生植物代表类群，利用可培养与免培养技术，结合形态、分子和高通量测序手段，揭示这一地区水生植物内生真菌的多样性、分布格局及其形成机制，检测大尺度下驱动水生植物内生真菌多样性格局形成的环境因子，为深入探讨内生真菌与植物的协同进化提供新的视野与证据，同时，为利用水生植物修复受损水生生态系统提供潜在的技术支持。

Endophytic fungus play important role in increasing biomass of plant and tolerance of plant to environment pressure. Many studies focused on endophytic fungus of terrestrial plants, and gave few attention to endophytic fungus of aquatic plants which have broader distributions. Limited research show that endophytes of aquatic plants embrace higher biodiversity and differ from those in terrestrial species, which also are important in helping plant resist to environment pressure. What’s more, aquatic plants are a good model in surveying impact of geographic patterns and environmental gradients on biodiversity, and studies on distribution pattern of endophytic fungus in aquatic plants will help further explore biodiversity.. On the basis of previous researches, we will study some representative species of aquatic plants in the Southwest region of China from following aspects: using culture-based and culture-independent technology, and integrating morphology, molecular characters and High-throughput sequencing. This project aims: (1) to explore the endophytic fungus diversity of aquatic plants, distribution pattern and its formation mechanism; (2) to analyse factors driving distribution pattern of endophytic fungus in a bigger scale, and test the hypothesis whether these regulations in terrestrial plants are available for aquatic plants; (3) to provide the new insight and evidence for endophyte biodiversity and coevolution between plant and fungus; (4) to promote the advance of using aquatic plants influenced by endophytic to restore the damaged aquatic ecosystems.