云南省石漠化面积居全国第二，石漠化地区的生态修复刻不容缓。大规模的田间实验显示，土壤接种剂在控制早期植物群落形成、决定植被类型、增加植物物种多样性、加速群落演替中均起着重要作用。植物种子是土壤接种剂的重要组成部分，也是决定土壤接种剂能否成功进行生态修复的关键。而种子内生真菌在种子保存、萌发以及植物抵抗环境压力的过程中又发挥着重要作用，但种子内生真菌多样性的调查在国内外还未系统开展，其在土壤接种剂中的作用也还未见报道。. 本项目将选择云南几个典型的石漠化地区，利用可培养和免培养技术，调查其周边地区优势物种种子内生真菌的组成及分布格局，揭示“植物王国”部分种子内生真菌的多样性，并探索形成其格局的驱动因子。同时，评估优势内生真菌在种子萌发中的作用，为利用土壤接种剂进行石漠化区域生态修复提供潜在的技术支持。

The area of rocky desertification in Yunnan Province ranks second in China, therefore, the ecological restoration in rocky desertification areas is an urgent task. Large-scale field experiments have shown that soil inoculants play an important role in controlling plant community formation of early stage, determining vegetation types, increasing plant species diversity, and accelerating community succession. Plant seeds are a core component of soil inoculants, and also are the key to determining whether soil inoculants can successfully repair ecological environment. The seed endophytic fungi plays an important role in seed preservation, germination and plant resistance to environmental stress, but the investigation of seed endophytic fungi diversity has not been systematically carried out at home and abroad, and its role in soil inoculants has not been reported yet.. The composition and distribution pattern of endophytic fungi in dominant plant species will be investigated surrounding several typical rocky desertification areas being selected in Yunnan Province in our project by using cultivable and culture-free techniques, which can reveal endophytic fungi diversity of some seeds in the “plant kingdom”, and explore the relevant driving factors for the formation of its patterns. At the same time, it also evaluates the role of endophytic fungi in seed germination, and provides potential technical support for the use of soil inoculants in relation to ecological restoration of rocky desertification areas.