进入新世纪，农业废弃物等资源高效转化形成高附值的功能食品原料、食品添加剂及生物基化学品已成绿色生物技术发展的重要方向，而嗜热微生物分泌的酶系热稳定性好、生产应用成本低而被广泛关注。本课题拟用整合组学的方法调研重要嗜热微生物基因组中碳水化合物活性酶的种类与数量，分析其降解模式与降解潜能；利用功能蛋白组学技术分析相关酶系高效分泌的条件与环境影响因素，阐明嗜热微生物胞外酶系中核心酶组分的种类与浓度变化、辅助酶组分高效诱导因素以及其辅助因子等增效功能；利用荧光辅助糖电泳（FACE）结合糖质谱等糖代谢组技术分析检测中间代谢种类与数量，明确不同酶系降解形成寡糖的产物谱特征与其高效转化的限速瓶颈；基于网络生物学技术系统分析降解代谢动态网络，在系统生物学水平上认识相关嗜热微生物降解多糖的偏好性，了解其嗜热环境下酶系的高效分泌机制，为功能酶系定制形成绿色高效转化工艺奠定理论与技术基础。

In the new era, it has gradually become the theme of green biotechnology development that efficiently converting agricultural waste resources to functional food ingredients, additional agent and bio-based chemicals with high attached value, while thermophilic microorganisms have been widely concerned due to the strong ability to secrete enzymes possessing a good thermal stability, long half-life and low production cost. In this study, the amount and type of carbohydrate active enzymes in the thermophilic microbial genome, the degradation potential and preference of specific biomass are investigated by integrated-omics. With functional proteomics technology, it is expected to analyze the environmental influence factor of efficient secreted enzymes, clarifying the composition and concentration changes of extracellular enzymes of thermophilic microorganisms, efficient induced factors of auxiliary enzyme components and their synergistic functional cofactors. Also, combining fluorophore-assisted carbohydrate electrophoresis with sugar metabonomics technology, the diversity of intermediate metabolites and the restriction factors of high-efficiency bioconversion can be determined. Furthermore, with the network biology technology, it is expected to explore the mechanism of efficient secretion of the microbial extracellular glycoside hydrolase under different carbon source culture conditions and the preference for biomass, which provide theoretical and technical basis for the optimization of fermentation technology in industry.