多囊卵巢综合征（PCOS）是育龄女性常见的生殖内分泌及代谢紊乱临床症候群。运动对改善代谢和促进PCOS患者排卵及月经周期恢复有积极效应，但机制不明。介导运动积极效应的因子irisin有明确改善代谢的作用，我们前期研究初步发现irisin除可改善PCOS小鼠代谢紊乱外，对其卵巢生殖内分泌功能也可能存在直接的调节作用。由于一些代谢调节因子在影响代谢的同时也参与生殖功能的调节，因此我们推测irisin也可能直接参与了调节卵泡发育过程，从而介导运动对PCOS患者排卵障碍的改善作用。目前尚缺乏这方面的研究，而对其研究不仅可加深我们对irisin的进一步认识，也可为PCOS的治疗提供新的思考方向。为此，本项目拟以PCOS小鼠模型、irisin及其受体的条件性敲除小鼠模型及小鼠腔前卵泡的体外培养为基础，在体外和体内两个层面获irisin调节PCOS小鼠卵巢生殖内分泌功能的效应和和分子机制。

Polycystic ovary syndrome（PCOS） is a common clinical syndrome of reproductive endocrine and metabolic disorders in women of childbearing age. Exercise has a positive effect on improving metabolism and promoting ovulation and menstrual cycle recovery in patients with PCOS, but its mechanism is unknown. Irisin has a explicit effect on improving metabolism as a factor mediating the positive effects of exercise. We have initially found that irisin might have a direct regulating effect on the ovarian reproductive endocrine function in addition to improving metabolic disorders in PCOS model mice. Because some metabolic regulatory factors may also participate in the regulation of reproductive functions while regulating metabolism, we speculate that irisin might directly participate in the process of regulating follicular development, ovulation, and hormone synthesis, thus mediating the improvement of ovulation disorders in PCOS patients. There is no report on this topic upuntil now,while it's investigation will not only provides a further understanding of the physiological effects of irisin, but also provides new thinking directions for the pathogenesis and treatment of PCOS. Therefore, this research, in which the conditional knockout mouse model of irisin receptor, PCOS mouse model, culture of anterior follicle in vitro was used, and irisin grouping, is to obtained the characteristics and molecular mechanism of irisin regulating reproductive endocrine function in the ovary of PCOS mice，and in order to provide new strategies for the treatment of PCOS ovulation disorders.