利用DNA分子鉴定技术可以实现中药材及其成药当中原料药的真伪鉴定，但是由于现有的中药基因检测方法存在操作流程复杂、不能进行定量分析等问题，中药基因检测技术在中药的质量控制过程中并未发挥出其应有的作用。申请人在前期研究过程中建立了一种快速核酸扩增方法（Ultrafast-VPCR），利用该方法结合新型的DNA快速提取试剂，可以将整个基因检测的时间从现在的4-7小时缩短至30分钟以内。本课题针对半夏药材及其成药掺假严重的情况，首先以半夏药材为研究对象，对半夏及其常见伪品的ITS、matK、rbcL等序列进行分析、设计半夏药材的特异性引物以及定量分析的探针，再与Ultrafast-VPCR相结合，建立半夏药材及其成药当中原料药的快速定性定量分析体系，从而实现对半夏药材及其成药的质量评价。同时，本项目可以推广到其他中药品种，为中药的质量控制提供新的思路和方法。

DNA-based techniques have proved to be effective way to identify species in Chinese herbal medicines and Chinese patent medicines. But these techniques haven’t played an important role in the quality control of Traditional Chinese medicine due to the time-costed process and lack of the ability to quantify the samples. A fast DNA amplification method （Ultrafast-VPCR）was established in our previous study. The gene detection time can be reduced from 4-7 hours to less than 30 minutes by taking advantage of the Ultrafast-VPCR and rapid DNA extraction regents. As Pinellia ternata related adulterations are very common in the market, a novel fast DNA identification system will be introduced in this project. Firstly, specific primers and probes of Pinellia ternata will be designed according to the alignment results of Pinellia ternata and its adulterations in the ITS、matK、rbcL sequences. Then the developed qualitative and quantitative identification system will be combined with Ultrafast-VPCR to establish a fast detection system for Pinellia ternata crude drugs and Pinellia ternata contained Chinese patent medicines. Meanwhile, this technique can also provide a novel idea and a new way for quality control of other kinds of Traditional Chinese medicine.