水稻地方品种月亮谷单粒传纯系互作诱导抗稻瘟病的机制研究

元阳梯田因其丰富的遗传多样性维持了数百年的持久抗性。以月亮谷为代表的地方品种多为农艺性状相似的混杂群体，其种内极高的遗传异质性是元阳梯田经久不衰的关键因素。然而，月亮谷种内植株相互作用应答诱导调控水稻抗性的机制还不清楚。前期研究发现，月亮谷不同纯系混栽能够显著提高植株自身的防御水平，从而进一步增强水稻对稻瘟病的抗性，因此推测纯系混栽能够通过化学互作和信号转导诱导调控水稻的抗性。本项目拟在此基础上，利用94个已完成全基因组测序的月亮谷单粒传纯系，基于多组学研究手段，围绕月亮谷纯系混栽通过改变水稻植株体内代谢及转录变化进而诱导调控植株对稻瘟病抗性的机制开展研究，结合GWAS分析和CRISPR技术，深入挖掘参与此诱导过程的关键基因，阐明月亮谷纯系混栽诱导调控植株抗性的作用原理，进而揭示元阳梯田持久抗病的化学生态学机制，为利用遗传多样性控制作物病害，构建现代农业可持续生态控病技术提供科学依据。

Yuanyang terraces have maintained durable resistance for hundreds of years due to the high genetic diversity. Most landraces represented by Acuce are population with similar agronomic traits rather than pure varieties. The extremely high genetic heterogeneity within the species is the key factor for the long-lasting resistance of the terraces. However, the mechanism of intraspecific interaction induced resistance to rice blast in mixed planting system is still unclear. Previous studies showed that rice mixed planting can significantly increase plant immunity and further enhance plant resistance to rice blast. Therefore, it is speculated that plant resistance could be induced in mixed planting system through allelopathic interaction and signal transduction. Our study intends to reveal the mechanism of plant-plant interaction induced-resistance to rice blast in the Acuce traditional population, based on multi-omics method and 94 sequenced Acuce lines (single seed descend). Combining with GWAS analysis and CRISPR technology, we would further explore the key genes involved in this induction process. And the mechanisms of induced-resistance in Acuce population will be elucidated. Revealing the chemical and ecological mechanism of durable resistance of Yuanyang terraces could provide a scientific basis for the use of genetic diversity to control crop diseases and for constructing sustainable and ecological technologies for disease control in modern agriculture.