表皮角质层在果实采后品质保持和抵御机械、微生物侵害等方面具有重要作用。CNR(Colorless Non-Ripening) 作为番茄SPL家族关键的果实品质调控转录因子，其直接参与的生物学过程及调控的靶基因网络仍缺乏系统研究。我们前期研究发现，CRISPR/Cas9技术获得CNR基因敲除番茄果实（C-CNR）角质层变厚、采后失水减弱、角质层相关基因表达升高，预示CNR影响表皮角质层特性。因此，本项目拟以CNR敲除和CNR过表达番茄果实为研究对象，系统分析CNR有无对表皮角质层显微结构、代谢物组份及含量、基因表达谱的影响；通过ChIP-seq筛选CNR靶向的角质层特性相关基因，体内体外验证其结合和转录活性，并验证靶基因功能。研究结果从形态学、代谢物、角质层相关靶基因多层面，阐明CNR对表皮角质层特性的分子调控机制，既丰富了果实角质层转录调控网络，又为果实采后保鲜理论研究提供了新思路。

Epidermal cuticle plays an important role in post-harvest quality maintenance and resistance to mechanical and microbial damage. Colorless Non-Ripening (CNR) is the key SPL family transcription factor during tomato fruit ripening. However, molecular regulation mechanism in the specific biological process and its direct target genes is still uncertain. In our previous study, CRISPR/Cas9 mediates CNR gene knockout tomato fruits (C-CNR) show thickened cuticle, decreased postharvest water loss and increased expression of cuticle related genes, indicating that CNR may affect the function of cuticle. In this project, the effect of CNR on fruit cuticle structure, cuticle deposition, cutin/wax/phenols component and content, and related genes expression level was carried out by using C-CNR and CNR-overexpressed fruits. Combined with ChIP-seq data to identify CNR direct regulated genes in cuticle formation and verify their function will help to dissect the molecular regulation network of CNR on the characteristics of tomato fruit cuticle，and provided a new idea for the research on postharvest fruit quality control.