碳纳米材料（CNMs）的广泛应用导致其不断进入水环境，并与内分泌干扰物（EDCs）等构成水体复合污染，对生态系统健康构成潜在威胁。目前这些新型污染物在水环境中的复合污染研究仍存在不足。本研究拟采用同位素示踪结合质谱分析技术，解析水体中典型CNMs与EDCs在水生生物体的分布、累积和代谢物生成，阐释CNMs对EDCs代谢转化途径的影响；利用生物体抗氧化、代谢和内分泌等功能在细胞和分子水平上的生物标志物响应，揭示两者复合污染的联合毒性效应和交互作用机制；分析水动力和水质条件对复合污染交互作用的影响，识别主要影响因素。该研究的结果可为评价复杂水体污染的生态健康效应提供必要的理论依据与技术支撑。

Due to the increasing use in various industries, carbon nanomaterials (CNMs) have inevitably released into the aquatic environment and coexisted with other emerging contaminants (eg. endocrine disrupting chemicals, EDCs) to form combined pollution, leading to an unpredictable health threat to ecosystem. However, there is little investigation about those emerging contaminants combined pollution. This project plans to analyze the distribution, accumulation and metabolism of CNMs and EDCs combined pollution in aquatic organisms using the methods of isotope tracer and mass spectrometry, and indicate the effects of CNMs on EDCs’ metabolic transformation. The biomarkers of antioxidation, metabolism and endocrine at the cell and molecular level will also be determined to reveal the joint toxicity and interactive mechanism of the combined pollution. The effects of environmental factors (eg. hydrodynamic factor and water quality) obtained from field researches will be also considered in the interaction, and the main factors will be identified. The results of project can be used to evaluate the ecological health of complex pollution in waters.