Long-term effects of antibiotics, norfloxacin, and sulfamethoxazole, in a partial life-cycle study with zebrafish (Danio rerio): effects on growth, development, and reproduction
Long-term effects of antibiotics, norfloxacin, and sulfamethoxazole, in a partial life-cycle study with zebrafish (Danio rerio): effects on growth, development, and reproduction
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斑马鱼（Danio rerio）部分生命周期研究中抗生素、诺氟沙星和磺胺甲恶唑的长期影响：对生长、发育和繁殖的影响
DOI：
10.1007/s11356-016-7018-1
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发表时间：
2016-06
影响因子：
5.8
通讯作者：
Liu Jianchao
中科院分区：
文献类型：
--
作者：
Yan Zhenhua;Lu Guanghua;Ye Qiuxia;Liu Jianchao
对斑马鱼（Danio rerio）进行了部分生命周期研究，以评估抗生素、诺氟沙星 (NOR) 和磺胺甲恶唑 (SMX) 的长期影响。评估了与生长、发育和繁殖相关的一系列生物终点。结果表明，200μg/L的SMX在生长期可降低体重和体况因子。同时，所有病例中代谢酶（乙氧基试卤灵O-脱乙基酶，EROD）和抗氧化酶（超氧化物歧化酶，SOD和过氧化氢酶，CAT）的活性均受到刺激。还研究了父母接触抗生素对下一代的影响。单独或联合使用 200 μg/L NOR 和 SMX 会抑制父母的产蛋量。同样，亲本暴露于最高浓度的 SMX 后，也会出现下一代孵化率、存活率下降和发育异常增强的情况。然而，在所有情况下心跳都没有改变。此外，在大多数情况下，联合治疗和单独治疗之间的生物终点没有显着差异，除了联合治疗中的 EROD 活性和产蛋量较低之外。结果表明，长期暴露于环境相关浓度的 NOR 和 SMX，无论是单独还是混合，可能不会对斑马鱼的生长、发育和繁殖构成显着威胁，并且在高浓度下可能会产生不利影响。
A partial life-cycle study with zebrafish (Danio rerio) was conducted to evaluate the long-term effects of antibiotics, norfloxacin (NOR) and sulfamethoxazole (SMX). A series of bio-endpoints correlated to the growth, development, and reproduction was assessed. The results showed that the body weight and the condition factor were depressed by SMX at 200 μg/L during the growth period. Meanwhile, the activities of metabolic enzyme (ethoxyresorufin O-deethylase, EROD) and antioxidant enzymes (superoxide dismutase, SOD and catalase, CAT) were stimulated in all cases. The consequences of parental exposure to antibiotics for the next generation were also examined. The egg production of parents were depressed by the 200 μg/L NOR and SMX alone or in combination. Similarly, decreased hatching, survival, and enhanced development abnormality of the next generation also occurred after parental exposure to SMX at the highest concentration. The heartbeat however was not altered in all cases. Furthermore, there was no significant difference in the bio-endpoints between the combined and individual treatment in most cases, with the exception of lower EROD activity and egg production in the co-treatment. The results suggest that long-term exposure to NOR and SMX at environmentally relevant concentrations, individually and in a mixture, may not significantly pose a threat to the growth, development, and reproduction of zebrafish, and an adverse effect may be expected at high concentration.
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DOI：
10.1080/15287394.2013.865107
发表时间：
2014-03-19
影响因子：
2.6
作者：
Melvin, Steven D.;Cameron, Mark C.;Lanctot, Chantal M.
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影响因子：
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