糖皮质激素在治疗临床多种难治病、慢性病过程中引发的药源性证候，是影响中医药疗效的新问题。既往学术界对皮质激素诱发人类肾虚证及其动物模型的研究停留于激素分泌及代谢产物层面，缺乏深入的机制探索。本研究提出“肾上腺皮质功能减退是糖皮质激素引起的药源性证候的根源，补肾中药通过提高肾上腺皮质细胞内胆固醇转化为类固醇激素的效率，从而发挥保护与恢复肾上腺皮质功能的作用”的科学假说。为此，我们将开展有关动物实验，模拟临床糖皮质激素用药方式制定标准化的动物造模方案，通过小鼠表征信息与反映肾上腺皮质功能客观指标，建立氢化可的松药源性证候的评价指标体系。进而通过常用补肾中药干预，观察对氢化可的松药源性证候小鼠肾上腺组织与原代培养的肾上腺皮质细胞胆固醇转化与合成类固醇激素的效率，以揭示不同补肾中药的分子药理作用新靶标，发现糖皮质激素药源性证候在肾上腺皮质功能层面的新机制，丰富中医肾虚证候理论。

The primary syndromes of drug-origin in the treatment of various stubborn and chronic diseases by glucocorticoid is a new problem that affects the efficacy of traditional Chinese medicine. In the past period of time, the study of kidney deficiency syndrome induced by glucocorticoid in human and experimental animals concentrating on hormones secretion and metabolites, however, the profound mechanism exploration is not enough. The hypothesis has been proposed in this project, hypocorticalism is the root cause of drug-induced syndrome induced by glucocorticoid, tonifying kidney herbs maybe improving the efficiency of the conversion from cholesterol to steroid hormones in adrenocortical cells, thus, the function of adrenal cortex can be protected or self recovery. Therefore, we will carry out animal experiments and expect to develop standardized animal modeling scheme by imitating clinical glucocorticoid medication way. Then, the evaluation index system of primary syndromes of drug-origin by hydrocortisone will be formulated by mice characterization informations and adrenal cortex function indexs. Then, the efficiency of cholesterol transformation and steroid synthesis in the adrenal tissue and primary cultured adrenal cortical cells of mice induced by hydrocortisone was observed with the intervention of tonifying kidney herbs. In order to reveal the new targets of molecular pharmacology of different tonifying kidney herbs, and to find the new mechanism of primary syndromes of drug-origin by glucocorticoid and enrich the theory of kidney deficiency syndrome in TCM.