椎间骨溶解涉及cage磨损颗粒特征与cage-宿主骨界面间细胞分子生物学反应，如何揭示该过程发生机理及寻求cage无菌性松动的治疗策略是脊柱外科界的研究热点。前期对仿生cage表面改性证实其具备稳定理化性征，促进骨化脂肪基质细胞定向粘附、增殖与分化，并验证组织工程学模式构筑新型活性cage的可行性。据种植体表面改性的研究表明，改性后材料具备调控细胞生长及基因表达的生物学效应。然而，基于表面改性后工程化cage能否消除磨损颗粒及下调炎性或破骨基因表达尚未阐明。因此，拟Nd:YAG+RGD联合修饰β-TCP/CS/PCL cage，复载羊骨化脂肪基质细胞，rhBMP-2,构筑工程化cage，观察骨溶解的生物学演变过程，研究RANK-RANKL-OPG信号通路相关的核心基因表达规律，以阐明磨损颗粒、cage表面性征、关键破骨基因间相互关系，为揭示椎间骨溶解机制及优化组织工程学治疗策略提供新思路。

Intervertebral osteolysis involves not only the wear particles of cage, but also the process of cellular or molecular reaction between cage-bone interface. The investigation of osteolysis mechanism and therapeutic strategy among cage loosening has been a challenging research topic in spinal surgery. We previously verified that the biomimetic spinal cage of surface modification could promote cellular affinity and proliferation of osteoinduced adipose derived stromal cells (ADSCs )with stable chemophysical characteristics, and demonstrated that the feasibility of constructing an novel biological cage via tissue-engineering approach.Additionally, surface-modified implant has been shown to regulate the cellular growth and gene expression with the effect of nanotography-induction. However, little has we known about whether tissue-engineered cage of surface modification could limit wear particles and downregulate inflamatory or osteoclastic genes.Therefore, we will plan to design the tissue-engineered cage construct via combining Nd:YAG laser with RGD modification and loading the goat's osteoinduced ADSCs with rhBMP-2 into the beta-TCP/CS/PCL cage.The aim of this study is to explore the biological reaction of spinal osteolysis, and to probe the expression pattern of RANK-RANKL-OPG signaling related genes between cage-bone interface, and elucidate the interrelationship between wear particles, cage surface characteristics and key reltaed osteoclastic genes during spinal osteolysis development.Our research will provide a new insight into the mechanism of interbody osteolysis and the therapeutic strategy of tissue-engineering.