减数分裂异常产生的2n配子在园艺植物种质创新和多倍体育种中具有巨大潜力。我们前期研究表明，香石竹DcPS1基因RNAi干扰后导致2n配子增多，花粉的育性下降，转录组数据发现RAD51D、CDC20和SKP1基因上调表达。拟南芥和水稻中RAD51D、CDC20和SKP1基因参与减数分裂过程，而在香石竹中DcPS1调控RAD51D、CDC20和SKP1基因参与减数分裂的机制还不清楚。本项目拟构建香石竹RAD51D、CDC20和SKP1基因CRISPR-Cas9载体和高表达载体并分别转化DcPS1基因的RNAi转基因植株和正常植株；通过免疫荧光反应等技术分析其在减数分裂过程的功能，采用RT-qPCR分析相关下游基因表达量的变化；利用酵母双杂交等技术研究RAD51D、SKP1和DcPS1相互作用，阐明DcPS1基因调控香石竹减数分裂和2n配子形成的分子机制，为香石竹多倍体遗传育种研究奠定基础。

2n gametes caused by abnormal meiosis have great potential for horticultural plant germplasm enrichment and polyploidy breeding. The results of our previous research showed that suppressing DcPS1 gene expression by double-stranded RNA-mediated interference (RNAi) in carnation had led to the increase of 2n gametes and the decrease of pollen fertility. Transcriptome data analyses had revealed expression of genes: RAD51D, CDC20 and SKP1 were up-regulated. In Arabidopsis and rice, genes: RAD51D, CDC20 and SKP1 were involved in meiotic processes, however, the mechanism how DcPS1 regulates RAD51D, CDC20 and SKP1 in carnation meiosis is still not clear. This project intends to construct CRISPR-Cas9 vector and over-expression vector of RAD51D, CDC20 and SKP1 gene in carnation which was transformed into DcPS1 gene RNAi transgenic plants and normal plants, respectively. The function of genes: RAD51D, CDC20 and SKP1 gene in meiosis will be analyzed by immunofluorescence reaction and the expression of downstream gene will be analyzed by RT-qPCR. The interacting proteins of RAD51D, SKP1 and DcPS1 will be identified using yeast two - hybrid system. The results will not only reveal the molecular mechanism of DcPS1 in regulating meiosis and 2n gamete formation, but also lay the foundation for the polyploid breeding of carnation.