The DcPS1 cooperates with OSDLa during pollen development and 2n gamete production in carnation meiosis.
The DcPS1 cooperates with OSDLa during pollen development and 2n gamete production in carnation meiosis.
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DcPS1 在花粉发育和康乃馨减数分裂中 2n 配子产生过程中与 OSDLa 合作
DOI：
10.1186/s12870-022-03648-z
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发表时间：
2022-05-24
影响因子：
5.3
通讯作者：
Yang, Xiaomi
中科院分区：
文献类型：
--
作者：
Zhou, Xuhong;Li, Shuying;Yang, Xiaomi
阐明减数分裂的机制对植物育种和物种进化具有重要意义。然而，对于康乃馨的减数分裂过程却知之甚少，康乃馨以其切花而闻名。我们报道了香石竹平行纺锤体1（DcPS 1）在花粉发育和香石竹减数分裂2n配子产生过程中调节第二次分裂的省略。在DcPS 1和OSDLa RNAi株系中，减数分裂第二次分裂的缺失和减数分裂第二次时纺锤体方向的异常可能是二分体/三联体形成的主要原因，导致未减数配子。我们还发现香石竹OSDLa与DcPS 1和DcRAD 51 D相互作用。在DcPS 1 RNAi株系中，观察到OSDLa和DcRAD 51 D表达的降低。在OSDLa RNAi株系中，还观察到DcPS 1和DcRAD 51 D表达的降低。我们建议，DcPS 1调节OSDLa的表达，允许进入减数分裂II和减数分裂II中的中期II纺锤体的正确方向。我们还提出，OSDLa调节DcRAD 51 D的表达，允许同源重组。这些结果表明DcPS 1和OSDLa在减数分裂过程中的双配子产生中起关键作用，并为减数分裂相关研究开辟了新途径。在线版本包含补充材料，可通过10.1186/s12870-022-03648-z获得。
Deciphering the mechanisms of meiosis has important implications for potential applications in plant breeding programmes and species evolution. However, the process of meiosis is poorly understood in carnation, which is famous for its cut flowers. We report that Dianthus caryophyllus parallel spindle 1 (DcPS1) regulates omission of second division like a (OSDLa) during pollen development and 2n gamete production in carnation meiosis. In DcPS1 and OSDLa RNAi lines, an absence of the second meiotic division and the abnormal orientation of spindles at meiosis II might be the main reason for dyad/triad formation, resulting in unreduced gametes. We also found that carnation OSDLa interacted with DcPS1 and DcRAD51D. In the DcPS1 RNAi lines, a decrease in OSDLa and DcRAD51D expression was observed. In the OSDLa RNAi lines, a decrease in DcPS1 and DcRAD51D expression was also observed. We propose that DcPS1 regulates OSDLa expression, allowing entry into meiosis II and the proper orientation of the metaphase II spindle in meiosis II. We also propose that OSDLa regulates DcRAD51D expression, allowing for homologous recombination. These results suggest a critical role for DcPS1 and OSDLa in diplogamete production during meiosis and open a new pathway for meiosis-related studies. The online version contains supplementary material available at 10.1186/s12870-022-03648-z.
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