银鲳在形态和运动上有其特殊性，无腹鳍，人工养殖一直是一个世界难题。在近十余年人工育苗关键技术有所突破后，才有了珍贵材料进行一些基础生物学的研究。本项目在已有研究成果的基础上，立足于综合转录组学、细胞生物学、免疫学和分子生物学等学科的方法和技术，研究miRNA对鳍发生相关靶基因tbx4、tbx5的调控机制，聚焦筛选在序列和表达模式上相互关联的miRNA和靶基因，融合高通量的RNA-seq测序、双荧光素酶报告基因检测、miRNAs过表达、抑制表达和形态学特征观察等策略，多方位解析miRNAs、靶基因tbx4、tbx5及其互作关系，验证miRNA 对相应基因的调控作用，推测其在银鲳腹鳍发生过程中的作用，初步探讨银鲳腹鳍发生模式变化的分子机理，为今后进一步解析银鲳进化过程中腹鳍丢失的分子机制提供理论依据。

Because of the special shape and movement of Pampus argenteu, no pelvic fins, artificial breeding of P. argenteu has always been a world problem. In the past more than ten years, as a breakthrough in the key technologies of artificial seedling, we have precious materials for some basic biological research. Combining the basis of previous research, and synthesizing transcriptome, cell biology, molecular biology, whole-mount in situ hybridization and whole-mount in situ immunofluorescence localization methods and techniques, the miRNA expression profiling and gene expression profiles of different developmental stages in P. argenteu are constructed by transcriptional genome sequencing; The miRNA expression profiling and gene expression profiles are analyzed comparatively by use of bioinformatics, and miRNA and target genes tbx4、tbx5 interrelated in sequence and expression pattern are screened; The cDNA of differential expression genes are cloned by the RT-PCR and rapid amplification of cDNA ends (RACE); Expression and location of miRNA and target genes tbx4、tbx5 are analyzed by Real-time PCR、Western Blotting, whole-mount in situ hybridization and immunofluorescence; Then combined with high-throughput RNA-seq sequencing, inhibition of expression or overexpression, double luciferase reporter test and morphological characteristics of P. argente development strategies, multi-directional analysis of miRNA and tbx4, tbx5，to verify the regulating effect of miRNA to target genes, and to speculate on its role in the development of P. argente ventral fin. Our aim is to explore the molecular mechanism of ventral fin occur pattern in P. argente, build technology platform of miRNA function analysis (identification and functional identification of target genes), provide a theoretical basis for further analysis of the molecular mechanism of pelvic fins loss during the evolution of P. argente.