Cloning and expression analysis of hepatic lipase from Pampus argenteus
Cloning and expression analysis of hepatic lipase from Pampus argenteus
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银鲳肝脂肪酶的克隆及表达分析
DOI：
10.1111/are.15008
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发表时间：
2020-11
影响因子：
2
通讯作者：
Danli Wang
中科院分区：
文献类型：
--
作者：
Shun Zhang;Gege Bao;Yu Zhou;Xuemei Yan;Shanliang Xu;Danli Wang
肝脂酶（HL）是血液循环中内源性甘油三酯代谢的关键酶。通过PCR和RACE技术克隆银鲳HL基因的全长cDNA，研究HL基因的分子机制和功能。采用实时荧光定量PCR和原位杂交技术研究HL在银鲳不同组织和生长阶段的时空表达特征及其在肝脏中的定位，并测定肝脏粗脂肪含量。银鲫HL基因cDNA全长为1836 bp（GenBank登录号KU 497716），开放阅读框（ORF）为1485 bp，编码494个氨基酸。银汉鱼HL蛋白与中华鲟HL蛋白同源性最高。在银鲫中，HL在肝脏中高度表达，在其他组织中几乎不表达。HL在银鲳不同生长阶段（1-5、5- 1 - 5、1 - 5 -30、30-50、50-100和>100 g）的表达先升高后降低，在30-50 g鱼重时表达量最高，与肝脂含量呈正相关。原位杂交结果显示，HL在肝细胞中的表达主要分布在细胞质和细胞核中，而在细胞核中不表达。
Hepatic lipase (HL) is a key enzyme in endogenous triglyceride metabolism in blood circulation. The molecular mechanism and function of Pampus argenteus HL gene was investigated by cloning the full-length cDNA of HL using PCR and RACE. The temporal and spatial expression characteristics of HL in different tissues and growth stages of P. argenteus and its localization in the liver were examined using real-time PCR and in situ hybridization, and liver crude fat content was determined. The full-length cDNA of P. argenteus HL gene was 1836 bp (GenBank No. KU497716); the 1485 bp ORF encodes 494 amino acids. P. argenteus HL protein had the highest homology with Siniperca chuatsi HL protein. In P. argenteus, HL was highly expressed in the liver and scarcely expressed in other tissues. HL expression in P. argenteus initially increased and then decreased at different growth stages (1-5, 5-15, 15-30, 30-50, 50-100 and >100 g), with the highest expression at 30-50 g fish weight, which was positively correlated with liver fat content. In situ hybridization showed that, in liver cells, HL was highly and weakly expressed in the cytoplasm and nucleolus, respectively, and not expressed in the nuclear interstitium.
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影响因子：
2
作者：
Huang, Yi-Sheng;Lin, Zhi-Deng;Wen, Xiao-Bo
通讯作者：
Wen, Xiao-Bo
DOI：
--
发表时间：
2010
期刊：
Journal of Jinan University
影响因子：
--
作者：
Huang Zhi-dong
通讯作者：
Huang Zhi-dong
DOI：
--
发表时间：
2010
期刊：
--
影响因子：
--
作者：
Yin Yayun
通讯作者：
Yin Yayun
DOI：
10.1074/jbc.m005436200
发表时间：
2000-10
期刊：
The Journal of Biological Chemistry
影响因子：
--
作者：
T. Ramsamy;T. Neville;B. Chauhan;Dhiraj Aggarwal;D. Sparks
通讯作者：
T. Ramsamy;T. Neville;B. Chauhan;Dhiraj Aggarwal;D. Sparks
DOI：
10.1016/j.cbpb.2006.06.008
发表时间：
2006-10
期刊：
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology
影响因子：
--
作者：
H. Oku;N. Koizumi;T. Okumura;Takanori Kobayashi;T. Umino
通讯作者：
H. Oku;N. Koizumi;T. Okumura;Takanori Kobayashi;T. Umino