棉蚜对氟啶虫胺腈抗性相关P450基因的鉴定及其表达调控机制

氟啶虫胺腈属砜亚胺类杀虫剂，对刺吸式口器害虫高效，广泛应用于我国棉蚜的防治。2015-2017年田间抗药性监测结果显示，我国棉蚜田间种群已经对氟啶虫胺腈产生了低等到中等水平的抗性。前期研究证明细胞色素P450介导了棉蚜对该药剂的代谢抗性。为明确P450在棉蚜对氟啶虫胺腈抗性中的作用及其调控机制，利用室内克隆的棉蚜敏感品系和氟啶虫胺腈抗性品系：1）通过比较转录组测序结合三代测序，鉴定抗性和敏感棉蚜中差异表达的P450基因，2）应用RNAi及转基因果蝇技术研究差异表达的P450基因在棉蚜对氟啶虫胺腈抗性中的作用，3）从表观遗传学（主要是miRNA）和转录因子调控角度研究抗性相关P450基因的表达调控机制。研究结果对于揭示棉蚜对氟啶虫胺腈抗性的分子机制，有效治理棉蚜的抗性具有重要的理论和实践意义。

Sulfoxaflor is the first commercially available sulfoximine insecticide that exhibits highly efficacy against many sap-feeding insect pests and has become an important insecticide against cotton aphids in China. Our monitoring results of insecticide resistance of Aphis gossypii from 2015 to 2017 indicated that the field populations collected from different regions of China had evolved low to moderate levels of resistance to sulfoxaflor. Previous studies demonstrated that cytochrome P450 mediated the metabolic resistance of A. gossypii to the sulfoxaflor. In the present study, a sulfoxaflor-resistant strain of A. gossypii established in the laboratory via continuous selection with sulfoxaflor was used to evaluate the role of cytochrome P450s in sulfoxaflor resistance in A. gossypii. 1) Identification of differentially expressed P450 genes through transcriptome and the 3rd generation sequencing; 2) Evaluation the roles of the differentially expressed P450 genes in sulfoxaflor resistance in A. gossypii by RNAi and bioassay with transgenic fruit fly overexpressed P450 genes of A. gossypii; 3) Investigate the mechanisms of expression regulation of sulfoxaflor resistance related P450 genes at transcriptional and post-transcriptional levels. The expected results will be useful to clarify the sulfoxaflor resistance mechanisms in A. gossypii and provide valuable information for management of sulfoxaflor resistant A. gossypii.