月季MLO基因家族中白粉病易感成员的筛选与功能鉴定

月季是世界第一大切花，也是我国广泛栽培的传统观赏植物。和其他园艺作物一样，月季生产中面临各种病虫害的侵扰，造成月季产量和品质的下降。其中月季白粉病是目前世界范围内月季生产所面临的最严重的毁灭性病害，也是我国优质、高效月季生产的重要限制因素之一。已有的研究表明，植物MLO （Mildew resistance Locus O） 基因家族中某些成员是植物对白粉病抗性的负调控因子，即易感基因，其功能丧失（隐性）突变可使植物获得对白粉菌的广谱、持久抗性，并在大麦等作物生产中获得了广泛的应用。 本项目拟对月季中的MLO基因家族进行系统的挖掘，对其序列特征、进化及亲缘关系进行分析，克隆其家族成员并分别验证其易感功能，分析其感病机制，以期初步阐明MLO家族成员调节月季对白粉病易感的分子机制，进而为月季抗病品质改良提供新的思路和基因资源。

Powdery mildew disease caused by Podosphaera pannosa is one of the most serious fungal threat to cut and pot rose production worldwide. It has been demonstrated that plant MLO (Mildew resistance Locus O) gene family is required for pathogenesis of powdery mildew fungi during their colonization in plants. As a susceptibility factor, loss-of-function mutations in MLO genes confer high levels of broad-spectrum resistance against powdery mildew in Barley and other plant species. The aim of this project is isolation and characterization of MLO homologs from rose. We will further investigate the involvement of candidate MLO genes in susceptibility towards P. pannosa. Collectively, our work will dissect the role of rose MLO genes in host- P. pannosa fungus interactions.