月季细胞表面受体RhWAK14介导灰霉病抗性的机制解析

月季是世界第一大切花，占全球切花贸易总额的三分之一以上。灰霉病是目前世界范围内月季采后所面临的最严重的毁灭性病害，也是我国优质、高效月季生产流通的重要限制因素之一。已有研究表明，植物细胞表面免疫受体WAK家族中某些成员可能参与包括灰霉病在内的死体营养型真菌的抗性。我们对月季中的WAK家族基因进行系统的挖掘，对其序列、亲缘关系及响应灰霉侵染的表达特征进行分析，分别验证其不同家族成员抗病功能，发现RhWAK14基因直接参与月季灰霉抗性发生。本项目进而对RhWAK14介导的灰霉抗性深入研究，分析其共受体、结合蛋白以及下游信号转导的关键元件，以期初步阐明RhWAK14为代表的WAK类细胞表面免疫受体调节月季灰霉抗性的分子机制，进而为月季抗病品质改良提供新的思路和基因资源。

Gray mold caused by air-borne pathogen Botrytis cinerea are among the most devastating diseases of rose worldwide. Recently, RhWAK14 gene responsible for resistance against Botrytis has been identified from rose. RhWAK14 encodes a cell wall-associated kinase that serves as a cell surface receptor for recognition of oligogalacturonides (OGs), which function as danger signals to induce the expression of defense genes and proteins, protecting plants against fungal diseases. In this project, several attempts will be made to understand RhWAK14-mediated defense signaling by identifying its co-receptors and other interacting proteins. Furthermore, using virus-induced gene silencing in rose petal, the signaling cascade downstream of RhWAK14 will be analyzed. These works will provide us a better understanding of RhWAKL14-mediated Botrytis defense signaling, and for WAKs signaling in general.