Comparative RNA-Seq analysis reveals a critical role for brassinosteroids in rose (Rosa hybrida) petal defense against Botrytis cinerea infection.
Comparative RNA-Seq analysis reveals a critical role for brassinosteroids in rose (Rosa hybrida) petal defense against Botrytis cinerea infection.
复制标题
比较 RNA-Seq 分析揭示了油菜素类固醇在玫瑰 (Rosahyda) 花瓣防御灰葡萄孢感染中的关键作用
DOI：
10.1186/s12863-018-0668-x
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发表时间：
2018-08-20
期刊：
影响因子：
2.9
通讯作者：
Zhang Z
中科院分区：
文献类型：
--
作者：
Liu X;Cao X;Shi S;Zhao N;Li D;Fang P;Chen X;Qi W;Zhang Z
背景：世界上最受欢迎的观赏植物之一，玫瑰（Rosa sp.），很容易感染灰霉病B.灰霉病会导致这些组织在生长和收获后阶段的崩溃和死亡，导致严重的经济损失。了解月季抗B的分子基础。结果：我们在B期间鉴定了花瓣中的差异转录基因（DTG）。接种后30 h（hpi）和/或48 h时的灰霉病感染。基因本体论术语富集和途径分析表明，代谢，次生代谢产物的生物合成，植物-病原体相互作用，植物激素信号转导途径。370细胞表面免疫受体的表达在感染过程中上调。此外，188个编码转录因子的基因表达上调，特别是在ERF、WRKY、bHLH、MYB和NAC家族中，这意味着它们参与了对B的抗性。灰绿色我们进一步确定了325上调DTG的激素信号转导途径。其中，油菜素类固醇（BR）相关基因的富集最为显著。为了证实BR在葡萄孢抗性中的作用，在接种B之前将外源BR施加到玫瑰花上。结论：我们的全球转录组分析提供了深入了解复杂的基因调控网络介导的玫瑰花瓣响应B。灰绿色我们进一步证明了植物激素BR在花瓣对坏死性真菌病原体的抗性中的作用。
Background:One of the most popular ornamental plants worldwide, roses (Rosa sp.), are very susceptible to Botrytis gray mold disease. The necrotrophic infection of rose petals by B. cinerea causes the collapse and death of these tissues in both the growth and post-harvest stages, resulting in serious economic losses. To understand the molecular basis of rose resistance against B. cinerea, we profiled the petal transcriptome using RNA-Seq technology.Results:We identified differentially transcribed genes (DTGs) in petals during B. cinerea infection at 30 h post inoculation (hpi) and/or 48 hpi. Gene ontology term enrichment and pathway analyses revealed that metabolic, secondary metabolite biosynthesis, plant-pathogen interaction, and plant hormone signal transduction pathways were involved. The expression of 370 cell-surface immune receptors was upregulated during infection. In addition, 188 genes encoding transcription factors were upregulated, particularly in the ERF, WRKY, bHLH, MYB, and NAC families, implying their involvement in resistance against B. cinerea. We further identified 325 upregulated DTGs in the hormone signal transduction pathways. Among them, the brassinosteroid (BR)-related genes were the most significantly enriched. To confirm the role of BR in Botrytis resistance, exogenous BR was applied to rose flowers before the inoculation of B. cinerea, which enhanced the defense response in these petals.Conclusions:Our global transcriptome profiling provides insights into the complex gene regulatory networks mediating the rose petal response to B. cinerea. We further demonstrated the role of the phytohormone BR in the resistance of petals to necrotrophic fungal pathogens.
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影响因子：
3.7
作者：
Moffat CS;Ingle RA;Wathugala DL;Saunders NJ;Knight H;Knight MR
通讯作者：
Knight MR
使用 RNA-Seq 组装具有超过 13,000 个全长表达基因的玫瑰转录组，并开发玫瑰 (Rosa L.) 的 WagRhSNP 68k Axiom SNP 阵列。
影响因子：
5.6
作者：
Koning-Boucoiran CF;Esselink GD;Vukosavljev M;van 't Westende WP;Gitonga VW;Krens FA;Voorrips RE;van de Weg WE;Schulz D;Debener T;Maliepaard C;Arens P;Smulders MJ
通讯作者：
Smulders MJ
影响因子：
11.6
作者：
Anderson, JP;Badruzsaufari, E;Kazan, K
通讯作者：
Kazan, K
影响因子：
7.4
作者：
Audenaert, K;De Meyer, GB;Höfte, MM
通讯作者：
Höfte, MM
影响因子：
64.5
作者：
Li, J;Wen, JQ;Walker, JC
通讯作者：
Walker, JC