污泥厌氧发酵主要包括产酸、产氢和产甲烷过程。课题组前期研究发现，污泥中的多环芳烃（PAHs）显著影响厌氧发酵产酸过程，中低剂量的PAHs均能够较大幅度提高污泥产酸效果。然而，PAHs是否影响污泥产氢产甲烷过程仍属未知。因此，本课题拟研究PAHs影响污泥产氢产甲烷的剂量-效应关系及其机理。首先，研究获得PAHs影响污泥产氢产甲烷的剂量-效应关系；然后从PAHs对产氢产甲烷相关过程，特别是对微生物种群、关键微生物酶活性、关键微生物基因转录和蛋白表达水平等影响的角度阐述其作用于污泥厌氧产氢产甲烷过程的机理；基于剂量-效应关系，进而研究获得调控PAHs污染水平以改善污泥厌氧产氢产甲烷效率的方法。这些研究将有助于人们深入认识PAHs影响污泥产氢产甲烷的规律及机理，开拓污泥厌氧生物转化和PAHs污染控制新的研究内容，对于实现污泥中能源物质的高效回收和PAHs污染防控具有重要的价值和意义。

Anaerobic fermentation of sludge mainly includes acetogenesis, hydrogenesis, methanogenesis. Previous studies found that polycyclic aromatic hydrocarbons (PAHs) significantly affected sludge acetogenesis, and under the conditions of moderate and low content the performance of organic acids production from sludge was remarkably enhanced by PAHs. However, till now whether PAHs influence sludge hydrogenesis and methanogenesis is still unknown. Thus, this project focuses on the relations between dosage and effect when PAHs affect sludge hydrogenesis and methanogenesis. Then, the mechanisms are explored from the aspects of PAHs giving impacts on the related processes for hydrogen and methane production, especially on microbial community, activities of key enzymes and levels of gene transcription and protein expression of key microorganisms. On the basis of relation between dosage and effect, the control methods for PAHs pollution level to improve the efficiency of anaerobic production of hydrogen and methane from sludge are achieved. These studies mentioned above will help people deeply understand the law and mechanisms of PAHs affecting hydrogen and methane production from sludge, and expand the new content about anaerobic bioconversion of sludge and PAHs pollution control, showing the important value and meaning for further realizing the efficient recovery of energy resource in sludge and prevention and control of PAHs pollution.