糖尿病创面增加社会医疗负担，影响生存质量。hADSCs分泌多种生长因子、局部分化为内皮细胞、神经细胞等，是治疗糖尿病创面的有效方法。而糖尿病hADSCs功能是否受损及机制仍不清楚。因此，本项目研究糖尿病hADSCs、高糖和糖基化终末产物处理后的hADSCs、转染MiR-210的hADSCs生物学特性，包括增殖和细胞周期，定量研究成骨、成脂、成内皮细胞分化，促内皮细胞迁移研究，自我更新基因和端粒酶活性检测，细胞因子阵列分析，微阵列芯片检测微小RNAs，RT-PCR检测HIF-1、ROS/AKT、PTPN2和PTP1B表达，同时研究上述hADSCs促裸鼠创面愈合的能力及机制。本项目将阐明糖尿病hADSCs功能受损的细胞分子机制，验证体外模拟糖尿病微环境的可行性，为恢复糖尿病hADSCs生物学功能，将自体hADSCs应用于糖尿病创面奠定理论基础。

Diabetic wound increased social and medical burden and affected the quality of life. hADSCs were an effective method for curing diabetic wound by secreting a variety of growth factors and differentiation into endothelial cells, nerve cells, etc.However, Whether biological function of diabetes hADSCs were damaged or not and the mechanism was still unclear.Hence,we will study biological activity of adipose-derived stem cells from diabetes mellitus, preprocessing with high sugar and glycosylation end products,transfection MiR-210,including proliferation and cell cycle, quantitative research of differentiation into osteoblast,adipose cells and endothelial cells, promoting endothelial cell migration, detection of self-renewal genes and telomerase activity, cytokines array analysis, detection of micro RNAs by microarray chip,detection of HIF-1,ROS/AKT,PTPN2 and PTP1B by RT-PCR , and to study the mechanism of accelerating wound healing of nude mice through hADSCs transplant. This study will clarify cell and molecular mechanism of impaired diabetic hADSCs and verify the feasibility of simulating diabetes microenvironment in vitro, and to establish theoretical foundation for restoreing biological function of diabetes hADSCs and transplanting autologous hADSCs to diabetic wound.