新型单链环状DNA病毒Gemykibivirus的病原特征和分子进化研究

Genomoviridae是一类有高多样性且来源广泛的小型环状单链DNA病毒，前期研究发现该类病毒在多种病例中出现。我们基于病毒宏基因组分析，首次报道了一个潜在的引起疾病的单链环状DNA病毒（GemyCN-GZ1），与发现于未知病因的人脑脊液和粪便样本中的几株病毒遗传关系接近；预实验显示该病毒具有一定的体外感染复制能力，这也暗示该病毒可能是能够对人进行潜在感染的新型病毒。目前该类病毒的病原特征如流行病学特点、感染复制能力和系统进化关系等仍是空白，因此我们将首先研究它的流行情况，进一步建立该病毒与疾病间的关系和风险；分析它与Gemykibivirus病毒的进化关系以及该类病毒在病例中的多样性特征，并通过基因进化特点研究其潜在传播风险以及与疾病的相关性；最后通过体外分离培养研究病毒的感染复制能力。本项目对进一步明确该病原的特点以及与疾病的关系有重要的基础和公共卫生意义。

Genomoviridae is a small circular single-stranded DNA virus with high diversity and wide source. Previous studies have found that this kind of virus appears in many cases, suggesting its potential risk of transmission and pathogenicity. Based on viral metagenomic analysis, we report for the first time a single-stranded cyclic DNA virus (GemyCN-GZ1) which can potentially cause human diseases (respiratory and brain infections). It is close genetically related to several viruses found in a few samples of human cerebrospinal fluid and feces with unknown etiology. Pre-experiments also showed that the virus had a certain ability to replicate in vitro infection, suggesting that the virus may be a new type of virus that can potentially infect people. Until now, the pathogenic characteristics of the virus, such as epidemiological characteristics, infectious replication ability and phylogenetic relationship, are still blank. Therefore, we will determine its epidemic situation in specific cases and further establish the relationship and risk between the virus and the disease. At the same time, we will analyze the evolutionary relationship between this virus and other Gemykibivirus and the diversity of Gemykibivirus in patients, through the evolutionary characteristics of genes we will determine the potential transmission risk of the virus and its correlation with diseases. Finally, the ability and mechanism of infection and replication of the virus were studied by in vitro isolation and culture. This project has important basic and public health significance for further clarifying the characteristics of the pathogen and its relationship with diseases.