CrAssphage and its bacterial host in cat feces.
CrAssphage and its bacterial host in cat feces.
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猫粪便中的 CrAssphage 及其细菌宿主
DOI：
10.1038/s41598-020-80076-9
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发表时间：
2021-01-12
影响因子：
4.6
通讯作者：
Delwart E
中科院分区：
文献类型：
--
作者：
Li Y;Gordon E;Shean RC;Idle A;Deng X;Greninger AL;Delwart E
CrAsA病毒是在人类粪便中检测到的一组不同的相关病毒，它们是最普遍和丰富的原核病毒。细菌的细胞宿主被鉴定为厌氧拟杆菌。在非人类灵长类动物和环境样本中也有报道，并已被提议作为人类粪便污染的标志。在这里，我们描述了猫粪便样本中的crAspherage DNA。在100 Kb左右的基因组中，有95%的基因组可以被组装到最近提出的Alphacrassvirinae亚科的1属中。通过部分线粒体DNA测序证实粪便样本的猫来源。在这只猫的粪便中也可以检测到高水平的拟杆菌DNA。在4周时间内纵向收集的粪便样品显示crAspherage DNA的连续脱落。因此，我们报告了第一个基因组序列确认的非灵长类哺乳动物的粪便样本中的crAseparage检测。
CrAssphages are a diverse group of related phages detected in human feces where they are the most prevalent and abundant prokaryotic virus. CrAssphages’ cellular host has been identified as the anaerobic Bacteroides intestinalis. CrAssphage has also been reported in non-human primates and environmental samples and has been proposed as a marker of human fecal contamination. Here we describe crAssphage DNA in a feline fecal sample. 95% of the ~ 100 Kb genome could be assembled and classified in genus 1 of the recently proposed Alphacrassvirinae subfamily. The cat origin of the fecal sample was confirmed by partial mitochondrial DNA sequencing. High levels of Bacteroides intestinalis DNA could also be detected in this cat’s feces. Fecal samples longitudinally collected over a 4-week period showed the continuous shedding of crAssphage DNA. We therefore report the first genome sequence-confirmed detection of crAssphage in fecal samples of a non-primate mammal.
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影响因子：
16.6
作者：
Siqueira JD;Dominguez-Bello MG;Contreras M;Lander O;Caballero-Arias H;Xutao D;Noya-Alarcon O;Delwart E
通讯作者：
Delwart E
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