Astrovirus Outbreak in an Animal Shelter Associated With Feline Vomiting.
Astrovirus Outbreak in an Animal Shelter Associated With Feline Vomiting.
复制标题
动物收容所爆发星状病毒，与猫呕吐有关
DOI：
10.3389/fvets.2021.628082
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发表时间：
2021
影响因子：
3.2
通讯作者：
Delwart E
中科院分区：
文献类型：
--
作者：
Li Y;Gordon E;Idle A;Hui A;Chan R;Seguin MA;Delwart E
在一个动物收容所观察到猫呕吐的爆发。检测已知的猫肠道病原体未发现致病因子。对来自同一地区的病例和对照的四个小型粪便池进行的病毒宏基因组学研究显示，所有池中都存在猫星状病毒。还发现在一个池中具有较少读段的是轮状病毒I、食肉动物博卡帕尔沃病毒3、诺如病毒（NoV）GVI和新型依赖病毒。高度流行的星状病毒的基因组被测序，并被分类为Mamastrovirus第二种，也被称为猫星状病毒。对11例患者纵向采集的粪便样本进行实时RT-PCR，结果显示10例（91%）患者脱落星状病毒长达19天。受影响的猫平均患病9.8天，中位数为2.5天（范围= 1-31天）。在爆发期间，在同一地区饲养的未受影响的对照猫显示，9只猫中有5只（56%）也在脱落星状病毒。从同一动物收容所约1年前（n = 8）和1年后（n = 10）采集的猫粪便样本显示，没有一个猫排出星状病毒，表明该病毒暂时与呕吐爆发相关，不是该收容所猫的肠道病毒组的一部分。再加上没有高度流行的已知病原体，我们的研究结果支持猫星状病毒感染的作用，以及显着的无症状脱落，在爆发传染性猫呕吐。
An outbreak of cat vomiting was observed in an animal shelter. Testing for known enteric feline pathogens did not identify a causative agent. Viral metagenomics on four mini pools of feces from cases and controls housed in the same area revealed the presence of feline astrovirus in all pools. Also found with fewer reads in one pool each were rotavirus I, carnivore bocaparvovirus 3, norovirus (NoV) GVI, and a novel dependovirus. The genome of the highly prevalent astrovirus was sequenced and classified into mamastrovirus species two, also known as feline astrovirus. Real-time RT-PCR on longitudinally acquired fecal samples from 11 sick cases showed 10 (91%) to be shedding astrovirus for as long as 19 days. Affected cats were sick for an average of 9.8 days, with a median of 2.5 days (range = 1–31 days). Unaffected control cats housed in the same areas during the outbreak showed five out of nine (56%) to also be shedding astrovirus. Feline fecal samples collected from the same animal shelter ~1 year before (n = 8) and after (n = 10) showed none to be shedding astrovirus, indicating that this virus was temporarily associated with the vomiting outbreak and is not part of the commensal virome for cats in this shelter. Together with the absence of highly prevalent known pathogens, our results support a role for feline astrovirus infection, as well as significant asymptomatic shedding, in an outbreak of contagious feline vomiting.
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DOI：
10.3390/v9050102
发表时间：
2017-05-10
期刊：
Viruses
影响因子：
--
作者：
Donato C;Vijaykrishna D
通讯作者：
Vijaykrishna D
影响因子：
5.4
作者：
Koci, MD;Moser, LA;Schultz-Cherry, S
通讯作者：
Schultz-Cherry, S
影响因子：
11.8
作者：
Boros Á;Albert M;Pankovics P;Bíró H;Pesavento PA;Phan TG;Delwart E;Reuter G
通讯作者：
Reuter G
DOI：
10.1093/bioinformatics/bts199
发表时间：
2012-06-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Kearse M;Moir R;Wilson A;Stones-Havas S;Cheung M;Sturrock S;Buxton S;Cooper A;Markowitz S;Duran C;Thierer T;Ashton B;Meintjes P;Drummond A
通讯作者：
Drummond A
影响因子：
9.4
作者：
Bouzalas, Ilias G.;Wuethrich, Daniel;Seuberlich, Torsten
通讯作者：
Seuberlich, Torsten