我国砷污染形势严峻，以砷超富集植物蜈蚣草为材料研发砷污染植物修复技术迫在眉睫，蜈蚣草砷转运分子元件的鉴定是该技术的主要瓶颈。本项目基于前期建立的蜈蚣草全长和差异转录组数据库，拟对转录水平砷广泛高度响应的八个初级主动转运蛋白ABC家族成员PvABCs进行系统的鉴定和解析：利用酵母功能互补体系初步确定具有砷转运能力的PvABCs成员；通过分子生物学、细胞生物学以及结构生物学等研究体系对砷转运PvABCs蛋白进行功能和结构解析，鉴定关键高效的砷转运PvABCs成员；进一步以之为分子元件，选择适生性强、生长迅速且生物量大的芒草作为“载体”，通过异源转化的手段构建工程植株，检测其砷富集能力，探讨其砷污染修复的应用前景。本项目将对阐明蜈蚣草砷转运分子机制具有重要意义，可为植物修复技术提供分子元件，为培育植物修复新型工程植株提供科学支撑。

The arsenic pollution is serious in China. It is urgent to develop the phytoremediation technology with the arsenic hyperaccumulator Pteris vittata. Identification of arsenic transporting molecular elements in this fern is the main bottleneck of the technology. Based on the ‘full-length transcriptomic and differential gene expression database’ in our previous study, this project aims to systematically identify and analyze PvABCs, members of the ABC family of eight primary active transporters with wide and high response to arsenic at transcriptional level. The function and structure of arsenic transporting PvABCs were analyzed by the study of molecular biology, cell biology and structural biology to idetify the key and efficient members of arsenic transporting PvABCs. Using the strong adaptability, rapid growth and large biomass plant Miscanthus as the "carrier", the engineering plant with the key and efficient members of arsenic transporting PvABCs will be constructed by heterologous transformation method and discussed about the application prospect in arsenic pollution phytoremediation. This study will not only contribute to understand arsenic transport mechanism in P. vittata, but also provide the molecular elements for phytoremediation techniques, and provide important references for the cultivation of new engineering plants for phytoremediation via biotechnology.