砷污染是全球环境热点问题之一，深入理解超富集植物砷富集分子机制是有效利用植物修复技术治理砷污染土壤的关键。本项目基于长测序读段的无参全长转录组测序技术，以砷超富集植物蜈蚣草为材料，拟对其超富集机制发挥重要作用的水通道蛋白家族进行功能成员的系统挖掘和深入解析。利用三代测序技术建立蜈蚣草全长转录组数据库；通过注释和比对获得水通道蛋白家族的基因序列，利用功能互补筛选体系鉴定具有砷转运能力的成员；对其在蜈蚣草中亚细胞定位、组织表达和砷响应模式等进行研究，绘制蜈蚣草砷转运水通道蛋白功能模式图。进一步对其成员组成、结构特性和家族演化进行分析，以期揭示具有砷转运能力的水通道蛋白其功能-结构-演化之间的关系。研究结果不仅对深入了解蜈蚣草砷超富集机制具有重要意义，而且可为培育新型植物修复工程植株挖掘基因和功能结构域等分子元件，为植物修复技术提供理论依据和科学支撑。

Environmental pollution caused by arsenic compounds in soil is important concern all around the world. Intensively study of the arsenic metabolism mechanism of hyper-accumulation plants is the key to control the arsenic contamination in soils. To identify the functional aquaporins (AQPs) integratively in P. vittata arsenic hyper-accumulation, the identification and characterization of PvAQPs based on the full-length transcriptome will be performed. The full-length transcriptome of P. vittata will be established using 3rd-generation transcriptome technology. PvAQPs will be identified by genomic annotation and homologous sequence alignment. The functional PvAQPs involved in arsenic transporting will be identified via the gene screen system. Characterization of the functional PvAQPs with the subcellular location, tissue expression and the regulation pattern by arsenic in P. vittata will be done to construct the diagram of the P. vittata arsenic transporting. The relationship among arsenic transporting function, the structure and evolution of PvAQPs will be revealed. This research will be of great importance in study the mechanism of P. vittata hyperaccumulation. Moreover, it will also provide the genetic components for the generation of phytoremediation engineering plant and the theoretical basis of phytoremediation technology.