珍稀濒危植物马蹄参的亲缘地理学和分子系统学研究

以濒危植物马蹄参（Diplopanax stachyanthus Hand. - Mazz.）为研究对象，通过测定核DNA和叶绿体DNA非编码区序列，以基因谱系方法研究其种群的遗传变异水平、遗传变异的分配状况和分化程度，探讨种群系统地理格局形成机制、系统发育关系和现有分布特征。根据核苷酸多样性、单倍型多样性和系统发育关系推测进化事件发生的时间。为保护和可持续利用该植物提供理论依据，为该植物遗传资源的保护和恢复提供基础资料。另外，由于该属植物系统位置存在争议，因此本项目拟筛选合适的序列，如nrDNA ITS、rbcL、trnL-F和trnS-G等，来分析五加科和山茱萸科植物尤其是木本植物的系统发育关系，期望借助分子系统学证据探讨马蹄参属植物的系统位置，同时结合形态和地理分布讨论该分类群的起源与系统演化关系。

Diplopanx stachyanthus, a rare species distributed across southern China will be investigated in this study. We aimed at addressing the questions on the formation of the genetic spatial patterns of the Diplopanx populations, and examining the phylogenetic relationship between taxa from Araliaceae and Cornaceae. The molecular population genetics approaches will by employed as described below. The coding and nonecoding genes including nrDNA, ITS, rbcL, trnL-F and trnS-G from the nuclear or chloroplast will be sequenced, to represent the genetic variation and genetic divergence among populations. The nucleotide polymorpholism and haplotype diversity, will be used in the phylogenetic infernces and divergence time estimation. This will not only help to clarify the phylogenetic relationship between the members from Araliaceae and Cornaceae, especially for the woody species, but also could shed light on the origin of the genera Diplopanx by integrating the molecular evidence and the information from morphology and distributional patterns. Our study will provide theoretical basis for conservation and sustainable exploitment, and additionally, for the conservation and recover of the genetic resources of the plants.