电压门控钙离子通道（VGCC）是一种镶嵌于细胞膜上的大分子蛋白复合体，由成孔、具有电压传感特性的α1亚基和辅助性β、α2/δ和γ亚基组成，介导细胞钙离子内流，在基因表达、神经递质的释放和肌肉收缩等生命活动中发挥重要的生理作用。目前对植物线虫VGCC的功能和药理学特性还知之甚少。为此，本研究拟对马铃薯腐烂茎线虫的VGCC各亚基基因进行克隆，通过原位杂交技术对VGCC各亚基的mRNA进行表达定位分析，并采用离体和半裸镰刀菌介导的活体RNAi技术对VGCC各亚基进行生物学功能分析；分别在HEK 293细胞异源表达L型、P/Q型和T型 VGCC，并在全细胞电压钳模式下测试各转染细胞系的电生理和药理学特性。研究结果有助于揭示植物线虫VGCC的结构和功能，为开发新型杀线虫剂提供理论基础。

Voltage-gated calcium channels (VGCC) are membrane protein complexes consisting of a pore-forming, voltage-sensing α1 subunit and other auxiliary subunits, such as β ,α2/δ and γ subunits. These channels provide the pathway for Ca2+ influxes that underlie a multitude of intracellular processes, such as gene regulation, neurotransmission and muscle contraction. However, in plant parasitic nematodes, little is known about their function and pharmacology properties. In this project, various VGCC subunits from Ditylenchus destructor will be cloned, and the expression location and biological function of VGCC subunits will be characterized by using mRNA in situ hybridization, in vitro RNAi and Fusarium semitectum mediated in vivo RNAi. L-, P/Q-, and T-type VGCC of D. destructor will heterogenously expressed in human embryonic kidney (HEK) 293 cells, and electrophysiological and pharmacological properties of transfected HEK293 cells will be observed using whole-cell patch voltage clamp technique. The results will add to our understanding of the structure & function of VGCC in plant parasitic nematode, support us to explore novel nematicides.